Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21751 | 5' | -54.1 | NC_004813.1 | + | 31212 | 0.69 | 0.57172 |
|
Target: 5'- --uCCGUCaguACCGcaauGGCCGaCACCUUCGCa -3' miRNA: 3'- auuGGCAG---UGGU----UUGGC-GUGGGAGUG- -5' |
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| 21751 | 5' | -54.1 | NC_004813.1 | + | 28070 | 0.66 | 0.736449 |
|
Target: 5'- gAACUG-CGCCAgcuuauaAGCCGCAUCCagCGCc -3' miRNA: 3'- aUUGGCaGUGGU-------UUGGCGUGGGa-GUG- -5' |
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| 21751 | 5' | -54.1 | NC_004813.1 | + | 25106 | 0.69 | 0.57172 |
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Target: 5'- -cACCGacaGCCAAACCGgUAUCCUCAa -3' miRNA: 3'- auUGGCag-UGGUUUGGC-GUGGGAGUg -5' |
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| 21751 | 5' | -54.1 | NC_004813.1 | + | 20751 | 0.66 | 0.768972 |
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Target: 5'- aGGCCGaacUCACCGGGagGCACCCggCAUc -3' miRNA: 3'- aUUGGC---AGUGGUUUggCGUGGGa-GUG- -5' |
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| 21751 | 5' | -54.1 | NC_004813.1 | + | 15841 | 0.66 | 0.779182 |
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Target: 5'- -uGCUGUCAUUuuuGCCGCGCgugCCUUACc -3' miRNA: 3'- auUGGCAGUGGuu-UGGCGUG---GGAGUG- -5' |
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| 21751 | 5' | -54.1 | NC_004813.1 | + | 15562 | 0.68 | 0.660847 |
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Target: 5'- -uGCUGUCGCCucauGCCGC-CCUUCu- -3' miRNA: 3'- auUGGCAGUGGuu--UGGCGuGGGAGug -5' |
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| 21751 | 5' | -54.1 | NC_004813.1 | + | 13561 | 0.71 | 0.455051 |
|
Target: 5'- -cACCGuUCGCCAGACCuuACCUUCGa -3' miRNA: 3'- auUGGC-AGUGGUUUGGcgUGGGAGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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