Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22236 | 5' | -57.7 | NC_005045.1 | + | 34507 | 0.66 | 0.501675 |
Target: 5'- gGCCacgaUGGcccACCGGCAGgGCGUCuucaacguuauGAAGg -3' miRNA: 3'- aCGGg---ACC---UGGUCGUCgUGCAG-----------CUUC- -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 41322 | 0.66 | 0.491233 |
Target: 5'- cGCCCUGGACCuauGCAaCGCccUGAAu -3' miRNA: 3'- aCGGGACCUGGu--CGUcGUGcaGCUUc -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 34472 | 0.66 | 0.491233 |
Target: 5'- gGCCgagCUGGugCucacGCGGCACGUUGccGg -3' miRNA: 3'- aCGG---GACCugGu---CGUCGUGCAGCuuC- -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 8832 | 0.66 | 0.489157 |
Target: 5'- cGCCCUGGACCcggaagagguucGCAagauGUACGcCGAGa -3' miRNA: 3'- aCGGGACCUGGu-----------CGU----CGUGCaGCUUc -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 34852 | 0.66 | 0.480893 |
Target: 5'- gGCCCUucgagugcuCCAGCAGUACGg-GAAGg -3' miRNA: 3'- aCGGGAccu------GGUCGUCGUGCagCUUC- -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 34218 | 0.66 | 0.480893 |
Target: 5'- gGCUgaUGGagaacuacGCCGGCAGUAUGUCGGGc -3' miRNA: 3'- aCGGg-ACC--------UGGUCGUCGUGCAGCUUc -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 16774 | 0.66 | 0.47066 |
Target: 5'- cGCUCUGGA--AGCAGaACGUCGGc- -3' miRNA: 3'- aCGGGACCUggUCGUCgUGCAGCUuc -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 7377 | 0.66 | 0.47066 |
Target: 5'- cGUCCUGGgauGCCAG-AGCAUGagcCGggGg -3' miRNA: 3'- aCGGGACC---UGGUCgUCGUGCa--GCuuC- -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 39663 | 0.66 | 0.464573 |
Target: 5'- aUGCCCUGGG-CGGCAcGCuuggcccguacauccACGUCGuGAGc -3' miRNA: 3'- -ACGGGACCUgGUCGU-CG---------------UGCAGC-UUC- -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 36022 | 0.67 | 0.44954 |
Target: 5'- aGCCCaGGGCaCGGCgcagcgccucauuGGCGgCGUCGAGu -3' miRNA: 3'- aCGGGaCCUG-GUCG-------------UCGU-GCAGCUUc -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 30683 | 0.67 | 0.44954 |
Target: 5'- cGCCCaccuggaUGGugCGGUugcGCACGUUGgcGg -3' miRNA: 3'- aCGGG-------ACCugGUCGu--CGUGCAGCuuC- -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 41075 | 0.67 | 0.43089 |
Target: 5'- gGCCCUGGaggugcguuauGCCAccuuGCAGCgccACGUCaaGGAGg -3' miRNA: 3'- aCGGGACC-----------UGGU----CGUCG---UGCAG--CUUC- -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 22848 | 0.68 | 0.393159 |
Target: 5'- aGCCCUGGAgUAGCGcaucaucCACcUCGGAGa -3' miRNA: 3'- aCGGGACCUgGUCGUc------GUGcAGCUUC- -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 22338 | 0.68 | 0.384067 |
Target: 5'- cGcCCCUGGcgagcACUAcggucGCGGcCACGUCGAGGa -3' miRNA: 3'- aC-GGGACC-----UGGU-----CGUC-GUGCAGCUUC- -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 25944 | 0.68 | 0.375115 |
Target: 5'- cGCUgagCUGGcCCGGCAgGCGC-UCGAAGg -3' miRNA: 3'- aCGG---GACCuGGUCGU-CGUGcAGCUUC- -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 37004 | 0.68 | 0.35764 |
Target: 5'- gGCCCUGGcAUUAGCcacaGGCGCcaCGAGGg -3' miRNA: 3'- aCGGGACC-UGGUCG----UCGUGcaGCUUC- -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 31937 | 0.69 | 0.340744 |
Target: 5'- aGCgCCUGGACCccauaGGUcccaGCGUCGAAGu -3' miRNA: 3'- aCG-GGACCUGGucg--UCG----UGCAGCUUC- -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 41947 | 0.69 | 0.306402 |
Target: 5'- gGCCUUGGcagucucgacagcaGCCAuCAGCuCGUCGAGGu -3' miRNA: 3'- aCGGGACC--------------UGGUcGUCGuGCAGCUUC- -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 28592 | 0.7 | 0.286227 |
Target: 5'- gGCCCUGGAUaucgAGCGGgAgGUCGgcGa -3' miRNA: 3'- aCGGGACCUGg---UCGUCgUgCAGCuuC- -5' |
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22236 | 5' | -57.7 | NC_005045.1 | + | 30894 | 0.7 | 0.279025 |
Target: 5'- cUGCCgCUGGugCGGUcGUAC-UCGAAGg -3' miRNA: 3'- -ACGG-GACCugGUCGuCGUGcAGCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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