Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22274 | 5' | -60.9 | NC_005045.1 | + | 41539 | 0.66 | 0.358268 |
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Target: 5'- cGGUGGCCUUggccuugaaucggCCUCGuCCAUGUCguguagggcgauGCGGu -3' miRNA: 3'- -UCGCCGGGA-------------GGAGCuGGUACGG------------UGCC- -5' |
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| 22274 | 5' | -60.9 | NC_005045.1 | + | 10793 | 0.66 | 0.376042 |
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Target: 5'- uGGauGUgCUCCUCGcucagGCCGguggcgGCCACGGu -3' miRNA: 3'- -UCgcCGgGAGGAGC-----UGGUa-----CGGUGCC- -5' |
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| 22274 | 5' | -60.9 | NC_005045.1 | + | 30177 | 0.66 | 0.384713 |
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Target: 5'- uAGCcucGCCUUCCUUGGCC--GCCugGa -3' miRNA: 3'- -UCGc--CGGGAGGAGCUGGuaCGGugCc -5' |
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| 22274 | 5' | -60.9 | NC_005045.1 | + | 12996 | 0.66 | 0.384713 |
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Target: 5'- uGgGGUCCUUCUUGAUg--GCCACGa -3' miRNA: 3'- uCgCCGGGAGGAGCUGguaCGGUGCc -5' |
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| 22274 | 5' | -60.9 | NC_005045.1 | + | 3318 | 0.66 | 0.384713 |
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Target: 5'- cGGCuuGGUCacagCCUCGACgAUuCCACGGg -3' miRNA: 3'- -UCG--CCGGga--GGAGCUGgUAcGGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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