Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22274 | 5' | -60.9 | NC_005045.1 | + | 34180 | 0.68 | 0.282589 |
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Target: 5'- cAGuCGGCCCUUgccguaCUUGACgGUGCCcugguCGGa -3' miRNA: 3'- -UC-GCCGGGAG------GAGCUGgUACGGu----GCC- -5' |
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| 22274 | 5' | -60.9 | NC_005045.1 | + | 38841 | 0.72 | 0.151807 |
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Target: 5'- uGGCcGUUCUCCUCGGCCuUGUCACcGGa -3' miRNA: 3'- -UCGcCGGGAGGAGCUGGuACGGUG-CC- -5' |
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| 22274 | 5' | -60.9 | NC_005045.1 | + | 40334 | 0.69 | 0.237113 |
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Target: 5'- gGGCGGCCCuugcgacgcUCC-CGGcCCAUGCCGa-- -3' miRNA: 3'- -UCGCCGGG---------AGGaGCU-GGUACGGUgcc -5' |
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| 22274 | 5' | -60.9 | NC_005045.1 | + | 41539 | 0.66 | 0.358268 |
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Target: 5'- cGGUGGCCUUggccuugaaucggCCUCGuCCAUGUCguguagggcgauGCGGu -3' miRNA: 3'- -UCGCCGGGA-------------GGAGCuGGUACGG------------UGCC- -5' |
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| 22274 | 5' | -60.9 | NC_005045.1 | + | 41809 | 0.68 | 0.262301 |
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Target: 5'- aAGCGGCCCUggguUCUggcuacaacgaGACCAaGCUGCGGg -3' miRNA: 3'- -UCGCCGGGA----GGAg----------CUGGUaCGGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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