Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22274 | 5' | -60.9 | NC_005045.1 | + | 13031 | 0.71 | 0.164512 |
Target: 5'- -uCGaGCCUgcaUCaugCGACCGUGCCACGGg -3' miRNA: 3'- ucGC-CGGG---AGga-GCUGGUACGGUGCC- -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 13581 | 0.69 | 0.219576 |
Target: 5'- cGCaGCCCUUgUUGACCucgGCgGCGGu -3' miRNA: 3'- uCGcCGGGAGgAGCUGGua-CGgUGCC- -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 33965 | 0.69 | 0.225296 |
Target: 5'- cGGCGucaGCCCggUCCa-GGCCAgggaUGCCACGGa -3' miRNA: 3'- -UCGC---CGGG--AGGagCUGGU----ACGGUGCC- -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 40334 | 0.69 | 0.237113 |
Target: 5'- gGGCGGCCCuugcgacgcUCC-CGGcCCAUGCCGa-- -3' miRNA: 3'- -UCGCCGGG---------AGGaGCU-GGUACGGUgcc -5' |
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22274 | 5' | -60.9 | NC_005045.1 | + | 3318 | 0.66 | 0.384713 |
Target: 5'- cGGCuuGGUCacagCCUCGACgAUuCCACGGg -3' miRNA: 3'- -UCG--CCGGga--GGAGCUGgUAcGGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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