Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22295 | 3' | -56.7 | NC_005045.1 | + | 37297 | 0.66 | 0.515212 |
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Target: 5'- aGCCUGUgGCUgaguuugGCAcUCGCGgcagucugucGUGGCGCg -3' miRNA: 3'- -CGGACGaUGA-------CGU-AGCGU----------CACCGCGg -5' |
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| 22295 | 3' | -56.7 | NC_005045.1 | + | 28463 | 0.66 | 0.523782 |
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Target: 5'- gGCCgcuaGCUACcGCAagcaagccguggccUCGCAgGUGGagGCCa -3' miRNA: 3'- -CGGa---CGAUGaCGU--------------AGCGU-CACCg-CGG- -5' |
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| 22295 | 3' | -56.7 | NC_005045.1 | + | 35520 | 0.66 | 0.537822 |
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Target: 5'- cCCUGCggcgccGCUGacgaUAUCGcCGGUGGCGgCu -3' miRNA: 3'- cGGACGa-----UGAC----GUAGC-GUCACCGCgG- -5' |
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| 22295 | 3' | -56.7 | NC_005045.1 | + | 33069 | 0.66 | 0.537822 |
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Target: 5'- cGCCUGC-GCUaCAgCGaCGGUGG-GCCg -3' miRNA: 3'- -CGGACGaUGAcGUaGC-GUCACCgCGG- -5' |
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| 22295 | 3' | -56.7 | NC_005045.1 | + | 14876 | 0.66 | 0.537822 |
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Target: 5'- cCCUGCUguaccgGCUGUccCGCAGgcaGUGCCu -3' miRNA: 3'- cGGACGA------UGACGuaGCGUCac-CGCGG- -5' |
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| 22295 | 3' | -56.7 | NC_005045.1 | + | 4496 | 0.66 | 0.537822 |
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Target: 5'- aCCgUGCUGC-GCAaCGacgGGUGGCGCa -3' miRNA: 3'- cGG-ACGAUGaCGUaGCg--UCACCGCGg -5' |
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| 22295 | 3' | -56.7 | NC_005045.1 | + | 34788 | 0.66 | 0.560763 |
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Target: 5'- gGCCUGCUuacCUGCGU-GCAGgaagcgauccaugaaGGUGUCc -3' miRNA: 3'- -CGGACGAu--GACGUAgCGUCa--------------CCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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