Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22295 | 3' | -56.7 | NC_005045.1 | + | 2207 | 0.68 | 0.41478 |
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Target: 5'- cGCCUGUUcaGCcuUGCGgaagaucggGCAGUcGGCGCCu -3' miRNA: 3'- -CGGACGA--UG--ACGUag-------CGUCA-CCGCGG- -5' |
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| 22295 | 3' | -56.7 | NC_005045.1 | + | 23306 | 0.69 | 0.349742 |
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Target: 5'- aGCCUGCUGCUggaucaccggguucgGCAucaccugcacaugguUCGgGGUcGGCGCg -3' miRNA: 3'- -CGGACGAUGA---------------CGU---------------AGCgUCA-CCGCGg -5' |
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| 22295 | 3' | -56.7 | NC_005045.1 | + | 5073 | 0.7 | 0.335483 |
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Target: 5'- aGCCUGg-GCUGCcgacgacuggacggCGCGGaUGGCGCUg -3' miRNA: 3'- -CGGACgaUGACGua------------GCGUC-ACCGCGG- -5' |
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| 22295 | 3' | -56.7 | NC_005045.1 | + | 41572 | 0.71 | 0.263704 |
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Target: 5'- cGCCgcccagggcagugGCUGCUGCgAUCacaGCGGUGGCcuugGCCu -3' miRNA: 3'- -CGGa------------CGAUGACG-UAG---CGUCACCG----CGG- -5' |
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| 22295 | 3' | -56.7 | NC_005045.1 | + | 21476 | 0.72 | 0.25558 |
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Target: 5'- cGCCUGCUGUUGCAUCaGuCGGUuuugcucuuccauacGGCGCg -3' miRNA: 3'- -CGGACGAUGACGUAG-C-GUCA---------------CCGCGg -5' |
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| 22295 | 3' | -56.7 | NC_005045.1 | + | 2918 | 0.75 | 0.146736 |
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Target: 5'- cGCCaUGUcgGCUGCGUUGCAGcGGCccGCCg -3' miRNA: 3'- -CGG-ACGa-UGACGUAGCGUCaCCG--CGG- -5' |
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| 22295 | 3' | -56.7 | NC_005045.1 | + | 34788 | 0.66 | 0.560763 |
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Target: 5'- gGCCUGCUuacCUGCGU-GCAGgaagcgauccaugaaGGUGUCc -3' miRNA: 3'- -CGGACGAu--GACGUAgCGUCa--------------CCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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