Results 21 - 29 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22306 | 3' | -57.4 | NC_005045.1 | + | 41716 | 0.67 | 0.43269 |
Target: 5'- aGCCCgCC-GGUGCGGUguacACGACCucGGu -3' miRNA: 3'- aCGGGaGGaCCGCGUCA----UGUUGGu-CC- -5' |
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22306 | 3' | -57.4 | NC_005045.1 | + | 27679 | 0.68 | 0.385774 |
Target: 5'- gGCCCUUCUGGauCAGaACcAUCAGGu -3' miRNA: 3'- aCGGGAGGACCgcGUCaUGuUGGUCC- -5' |
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22306 | 3' | -57.4 | NC_005045.1 | + | 18868 | 0.68 | 0.367972 |
Target: 5'- gUGCCCgcuuggUCUGGUGCuuGGUGCGgcguACCAGc -3' miRNA: 3'- -ACGGGa-----GGACCGCG--UCAUGU----UGGUCc -5' |
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22306 | 3' | -57.4 | NC_005045.1 | + | 40613 | 0.68 | 0.367972 |
Target: 5'- cUGCCUUCCagGGCGacaAGgcCGGCgAGGg -3' miRNA: 3'- -ACGGGAGGa-CCGCg--UCauGUUGgUCC- -5' |
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22306 | 3' | -57.4 | NC_005045.1 | + | 22271 | 0.69 | 0.359284 |
Target: 5'- gUGCCCUUCUuGCGCuGUACGuggguguaCAGGu -3' miRNA: 3'- -ACGGGAGGAcCGCGuCAUGUug------GUCC- -5' |
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22306 | 3' | -57.4 | NC_005045.1 | + | 5641 | 0.69 | 0.325988 |
Target: 5'- aUGCCCggcUCUUGGCcacGCGGcGCAucuCCGGGg -3' miRNA: 3'- -ACGGG---AGGACCG---CGUCaUGUu--GGUCC- -5' |
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22306 | 3' | -57.4 | NC_005045.1 | + | 9760 | 0.7 | 0.318029 |
Target: 5'- aUGCCCUCCaGGC-CAcgGCAGCCuacgAGGg -3' miRNA: 3'- -ACGGGAGGaCCGcGUcaUGUUGG----UCC- -5' |
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22306 | 3' | -57.4 | NC_005045.1 | + | 32821 | 0.74 | 0.155346 |
Target: 5'- gUGCCUacacgUCCUGGaGCGGUGCugccgcgaaccugGACCAGGg -3' miRNA: 3'- -ACGGG-----AGGACCgCGUCAUG-------------UUGGUCC- -5' |
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22306 | 3' | -57.4 | NC_005045.1 | + | 11272 | 0.66 | 0.529031 |
Target: 5'- uUGCUCUggcuguauuucugugCCUGGCGCAGcaUGCcauCCAGu -3' miRNA: 3'- -ACGGGA---------------GGACCGCGUC--AUGuu-GGUCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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