Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22306 | 3' | -57.4 | NC_005045.1 | + | 29297 | 0.7 | 0.310219 |
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Target: 5'- aUGCCCUacaggcgCUGGCGCAGcaGCAgaagGCCgAGGa -3' miRNA: 3'- -ACGGGAg------GACCGCGUCa-UGU----UGG-UCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 32224 | 0.68 | 0.367096 |
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Target: 5'- gGUgCUCUUGGCGCuggcgugGGUGCucuGCUGGGg -3' miRNA: 3'- aCGgGAGGACCGCG-------UCAUGu--UGGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 32821 | 0.74 | 0.155346 |
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Target: 5'- gUGCCUacacgUCCUGGaGCGGUGCugccgcgaaccugGACCAGGg -3' miRNA: 3'- -ACGGG-----AGGACCgCGUCAUG-------------UUGGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 35757 | 0.74 | 0.155778 |
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Target: 5'- cUGCCCaggUCCcGGacaCGCAGUuCAGCCAGGg -3' miRNA: 3'- -ACGGG---AGGaCC---GCGUCAuGUUGGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 36927 | 0.72 | 0.233863 |
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Target: 5'- gUGuCCCUCgUGGCGCcuGUgGCuaauGCCAGGg -3' miRNA: 3'- -AC-GGGAGgACCGCGu-CA-UGu---UGGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 38900 | 0.66 | 0.514117 |
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Target: 5'- gGgCCUCggGGCGCAgGUAC-GCCAGc -3' miRNA: 3'- aCgGGAGgaCCGCGU-CAUGuUGGUCc -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 40613 | 0.68 | 0.367972 |
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Target: 5'- cUGCCUUCCagGGCGacaAGgcCGGCgAGGg -3' miRNA: 3'- -ACGGGAGGa-CCGCg--UCauGUUGgUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 41197 | 0.67 | 0.452366 |
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Target: 5'- --aCCUCCUGGCGCuggguGUugGuCCuGGc -3' miRNA: 3'- acgGGAGGACCGCGu----CAugUuGGuCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 41716 | 0.67 | 0.43269 |
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Target: 5'- aGCCCgCC-GGUGCGGUguacACGACCucGGu -3' miRNA: 3'- aCGGGaGGaCCGCGUCA----UGUUGGu-CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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