Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22306 | 3' | -57.4 | NC_005045.1 | + | 4187 | 0.68 | 0.403205 |
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Target: 5'- gGCauaUUCCUGGCacuacguGCAGUA-GGCCGGGg -3' miRNA: 3'- aCGg--GAGGACCG-------CGUCAUgUUGGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 41716 | 0.67 | 0.43269 |
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Target: 5'- aGCCCgCC-GGUGCGGUguacACGACCucGGu -3' miRNA: 3'- aCGGGaGGaCCGCGUCA----UGUUGGu-CC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 41197 | 0.67 | 0.452366 |
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Target: 5'- --aCCUCCUGGCGCuggguGUugGuCCuGGc -3' miRNA: 3'- acgGGAGGACCGCGu----CAugUuGGuCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 5733 | 0.67 | 0.452366 |
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Target: 5'- aUGCCgaUCUagaGGCGCGGguaGCGGCCAGc -3' miRNA: 3'- -ACGGgaGGA---CCGCGUCa--UGUUGGUCc -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 22420 | 0.66 | 0.492077 |
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Target: 5'- aGUCCUCgacgUGGCcgcgaccGUAGUGCucGCCAGGg -3' miRNA: 3'- aCGGGAGg---ACCG-------CGUCAUGu-UGGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 27013 | 0.66 | 0.493117 |
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Target: 5'- cGUCCUCCUGuCGCAggaGUACGucugcaugucgGCCAGc -3' miRNA: 3'- aCGGGAGGACcGCGU---CAUGU-----------UGGUCc -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 38900 | 0.66 | 0.514117 |
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Target: 5'- gGgCCUCggGGCGCAgGUAC-GCCAGc -3' miRNA: 3'- aCgGGAGgaCCGCGU-CAUGuUGGUCc -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 8524 | 0.66 | 0.524753 |
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Target: 5'- cGCUCagCC-GGCGCAGcucaUAC-GCCAGGu -3' miRNA: 3'- aCGGGa-GGaCCGCGUC----AUGuUGGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 11272 | 0.66 | 0.529031 |
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Target: 5'- uUGCUCUggcuguauuucugugCCUGGCGCAGcaUGCcauCCAGu -3' miRNA: 3'- -ACGGGA---------------GGACCGCGUC--AUGuu-GGUCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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