Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22306 | 3' | -57.4 | NC_005045.1 | + | 13222 | 0.7 | 0.287665 |
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Target: 5'- gGCCggauggcaaCUGGCGcCAGUACcGCCGGGg -3' miRNA: 3'- aCGGgag------GACCGC-GUCAUGuUGGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 2865 | 0.71 | 0.252978 |
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Target: 5'- aGCCgaCaUGGCGCuGUGCGACCAuGGc -3' miRNA: 3'- aCGGgaGgACCGCGuCAUGUUGGU-CC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 36927 | 0.72 | 0.233863 |
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Target: 5'- gUGuCCCUCgUGGCGCcuGUgGCuaauGCCAGGg -3' miRNA: 3'- -AC-GGGAGgACCGCGu-CA-UGu---UGGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 15673 | 0.73 | 0.199275 |
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Target: 5'- -uUCUUCCUGGCGCAGUG--GCUGGGc -3' miRNA: 3'- acGGGAGGACCGCGUCAUguUGGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 18478 | 0.74 | 0.173938 |
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Target: 5'- cGCUCUUCUGGaCGCGGUGCuGCCcgaagAGGc -3' miRNA: 3'- aCGGGAGGACC-GCGUCAUGuUGG-----UCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 35757 | 0.74 | 0.155778 |
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Target: 5'- cUGCCCaggUCCcGGacaCGCAGUuCAGCCAGGg -3' miRNA: 3'- -ACGGG---AGGaCC---GCGUCAuGUUGGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 32821 | 0.74 | 0.155346 |
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Target: 5'- gUGCCUacacgUCCUGGaGCGGUGCugccgcgaaccugGACCAGGg -3' miRNA: 3'- -ACGGG-----AGGACCgCGUCAUG-------------UUGGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 11524 | 0.74 | 0.151513 |
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Target: 5'- gGCCUUCCUGcGaCGCGGUG--GCCGGGu -3' miRNA: 3'- aCGGGAGGAC-C-GCGUCAUguUGGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 8414 | 1.11 | 0.00028 |
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Target: 5'- aUGCCCUCCUGGCGCAGUACAACCAGGg -3' miRNA: 3'- -ACGGGAGGACCGCGUCAUGUUGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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