Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22306 | 3' | -57.4 | NC_005045.1 | + | 11272 | 0.66 | 0.529031 |
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Target: 5'- uUGCUCUggcuguauuucugugCCUGGCGCAGcaUGCcauCCAGu -3' miRNA: 3'- -ACGGGA---------------GGACCGCGUC--AUGuu-GGUCc -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 11108 | 0.68 | 0.385774 |
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Target: 5'- gGUCCccaUCCUGcaccuguCGCAGUGgGGCCGGGa -3' miRNA: 3'- aCGGG---AGGACc------GCGUCAUgUUGGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 9760 | 0.7 | 0.318029 |
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Target: 5'- aUGCCCUCCaGGC-CAcgGCAGCCuacgAGGg -3' miRNA: 3'- -ACGGGAGGaCCGcGUcaUGUUGG----UCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 8524 | 0.66 | 0.524753 |
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Target: 5'- cGCUCagCC-GGCGCAGcucaUAC-GCCAGGu -3' miRNA: 3'- aCGGGa-GGaCCGCGUC----AUGuUGGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 8414 | 1.11 | 0.00028 |
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Target: 5'- aUGCCCUCCUGGCGCAGUACAACCAGGg -3' miRNA: 3'- -ACGGGAGGACCGCGUCAUGUUGGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 5733 | 0.67 | 0.452366 |
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Target: 5'- aUGCCgaUCUagaGGCGCGGguaGCGGCCAGc -3' miRNA: 3'- -ACGGgaGGA---CCGCGUCa--UGUUGGUCc -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 5641 | 0.69 | 0.325988 |
|
Target: 5'- aUGCCCggcUCUUGGCcacGCGGcGCAucuCCGGGg -3' miRNA: 3'- -ACGGG---AGGACCG---CGUCaUGUu--GGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 4187 | 0.68 | 0.403205 |
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Target: 5'- gGCauaUUCCUGGCacuacguGCAGUA-GGCCGGGg -3' miRNA: 3'- aCGg--GAGGACCG-------CGUCAUgUUGGUCC- -5' |
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| 22306 | 3' | -57.4 | NC_005045.1 | + | 2865 | 0.71 | 0.252978 |
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Target: 5'- aGCCgaCaUGGCGCuGUGCGACCAuGGc -3' miRNA: 3'- aCGGgaGgACCGCGuCAUGUUGGU-CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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