Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22308 | 3' | -62.2 | NC_005045.1 | + | 9813 | 0.7 | 0.171622 |
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Target: 5'- cUGGCCCGAcccCGAaGGCGgccCGCgCCUGGg -3' miRNA: 3'- uGCCGGGUU---GCUcCCGCa--GCG-GGACC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 33527 | 0.7 | 0.176183 |
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Target: 5'- gAUGGCUgAGC-AGGGCGUUGgCCUGa -3' miRNA: 3'- -UGCCGGgUUGcUCCCGCAGCgGGACc -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 29907 | 0.7 | 0.185629 |
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Target: 5'- gGCGGCCCuggaAGCGGGGGacaucaaCGCCCUa- -3' miRNA: 3'- -UGCCGGG----UUGCUCCCgca----GCGGGAcc -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 40534 | 0.69 | 0.190517 |
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Target: 5'- gGCGGCugCCcuCGccGGCcuuGUCGCCCUGGa -3' miRNA: 3'- -UGCCG--GGuuGCucCCG---CAGCGGGACC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 30238 | 0.69 | 0.195519 |
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Target: 5'- -aGGCCgAGC-AGGGCGcCGUggCCUGGg -3' miRNA: 3'- ugCCGGgUUGcUCCCGCaGCG--GGACC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 37997 | 0.69 | 0.211215 |
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Target: 5'- gGCGGCCa---GAGGGCa--GCgCCUGGg -3' miRNA: 3'- -UGCCGGguugCUCCCGcagCG-GGACC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 30846 | 0.67 | 0.269505 |
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Target: 5'- aGCGGCagccuCCucgccgucgaguacGACGAGaacGGUGUgGCCCUGGa -3' miRNA: 3'- -UGCCG-----GG--------------UUGCUC---CCGCAgCGGGACC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 28590 | 0.67 | 0.292103 |
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Target: 5'- gUGGCCCuggauaucgAGCGGGaggucGGCGagGCCCUGa -3' miRNA: 3'- uGCCGGG---------UUGCUC-----CCGCagCGGGACc -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 9461 | 1.08 | 0.0002 |
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Target: 5'- cACGGCCCAACGAGGGCGUCGCCCUGGc -3' miRNA: 3'- -UGCCGGGUUGCUCCCGCAGCGGGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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