Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22321 | 5' | -53.2 | NC_005045.1 | + | 27171 | 0.72 | 0.39523 |
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Target: 5'- aGGACUUGAUCGUCuucGCCaaGAaguaucAGGCCGu -3' miRNA: 3'- -CCUGGACUAGUAGuu-CGG--CU------UCCGGU- -5' |
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| 22321 | 5' | -53.2 | NC_005045.1 | + | 27771 | 0.66 | 0.720614 |
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Target: 5'- gGGACCUGAUCAagGAuGCCucu-GCCGu -3' miRNA: 3'- -CCUGGACUAGUagUU-CGGcuucCGGU- -5' |
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| 22321 | 5' | -53.2 | NC_005045.1 | + | 28591 | 0.74 | 0.324569 |
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Target: 5'- uGGcCCUGGaUAUCGAGCgGGAGGUCGg -3' miRNA: 3'- -CCuGGACUaGUAGUUCGgCUUCCGGU- -5' |
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| 22321 | 5' | -53.2 | NC_005045.1 | + | 30209 | 0.66 | 0.753279 |
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Target: 5'- cGGcaACgUGAUgcUAUCAGGuaucCCGGAGGCCGa -3' miRNA: 3'- -CC--UGgACUA--GUAGUUC----GGCUUCCGGU- -5' |
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| 22321 | 5' | -53.2 | NC_005045.1 | + | 31646 | 0.66 | 0.76392 |
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Target: 5'- uGGGCC---UCGUCGAGCUGugcuuGGUCAa -3' miRNA: 3'- -CCUGGacuAGUAGUUCGGCuu---CCGGU- -5' |
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| 22321 | 5' | -53.2 | NC_005045.1 | + | 32563 | 0.74 | 0.300606 |
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Target: 5'- cGGCCUGA-CAggcugugcagcUCGcGCCGAAGGCCGu -3' miRNA: 3'- cCUGGACUaGU-----------AGUuCGGCUUCCGGU- -5' |
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| 22321 | 5' | -53.2 | NC_005045.1 | + | 33208 | 0.71 | 0.434096 |
|
Target: 5'- aGGGCCgUGucagCAUCGGaCCGGAGGCCc -3' miRNA: 3'- -CCUGG-ACua--GUAGUUcGGCUUCCGGu -5' |
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| 22321 | 5' | -53.2 | NC_005045.1 | + | 33774 | 0.67 | 0.698344 |
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Target: 5'- aGACCgUGAaucUCGUCuGAGCgGucGGCCAg -3' miRNA: 3'- cCUGG-ACU---AGUAG-UUCGgCuuCCGGU- -5' |
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| 22321 | 5' | -53.2 | NC_005045.1 | + | 41467 | 0.71 | 0.47509 |
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Target: 5'- -uACCUGGUagaagGUCAGGCCGGuguuacGGCCAg -3' miRNA: 3'- ccUGGACUAg----UAGUUCGGCUu-----CCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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