Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22321 | 5' | -53.2 | NC_005045.1 | + | 33774 | 0.67 | 0.698344 |
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Target: 5'- aGACCgUGAaucUCGUCuGAGCgGucGGCCAg -3' miRNA: 3'- cCUGG-ACU---AGUAG-UUCGgCuuCCGGU- -5' |
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| 22321 | 5' | -53.2 | NC_005045.1 | + | 10064 | 0.67 | 0.702823 |
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Target: 5'- gGGuACgUGAUCGUCGAGCCuAAGaucgacgggugccguGCCAu -3' miRNA: 3'- -CC-UGgACUAGUAGUUCGGcUUC---------------CGGU- -5' |
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| 22321 | 5' | -53.2 | NC_005045.1 | + | 27771 | 0.66 | 0.720614 |
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Target: 5'- gGGACCUGAUCAagGAuGCCucu-GCCGu -3' miRNA: 3'- -CCUGGACUAGUagUU-CGGcuucCGGU- -5' |
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| 22321 | 5' | -53.2 | NC_005045.1 | + | 22148 | 0.66 | 0.730518 |
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Target: 5'- aGGACCUGAUgCGcgCAGGCCGcAaccuguccgguucGGGCa- -3' miRNA: 3'- -CCUGGACUA-GUa-GUUCGGC-U-------------UCCGgu -5' |
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| 22321 | 5' | -53.2 | NC_005045.1 | + | 23927 | 0.66 | 0.742506 |
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Target: 5'- uGGCCUGggCAUCGcccgaguccaucAGCCGAccgauggcGGCCu -3' miRNA: 3'- cCUGGACuaGUAGU------------UCGGCUu-------CCGGu -5' |
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| 22321 | 5' | -53.2 | NC_005045.1 | + | 4127 | 0.66 | 0.753279 |
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Target: 5'- aGGcGCCUGc-CAgCAGGCCGAggcagcaguacAGGCCGa -3' miRNA: 3'- -CC-UGGACuaGUaGUUCGGCU-----------UCCGGU- -5' |
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| 22321 | 5' | -53.2 | NC_005045.1 | + | 30209 | 0.66 | 0.753279 |
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Target: 5'- cGGcaACgUGAUgcUAUCAGGuaucCCGGAGGCCGa -3' miRNA: 3'- -CC--UGgACUA--GUAGUUC----GGCUUCCGGU- -5' |
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| 22321 | 5' | -53.2 | NC_005045.1 | + | 31646 | 0.66 | 0.76392 |
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Target: 5'- uGGGCC---UCGUCGAGCUGugcuuGGUCAa -3' miRNA: 3'- -CCUGGacuAGUAGUUCGGCuu---CCGGU- -5' |
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| 22321 | 5' | -53.2 | NC_005045.1 | + | 21160 | 0.66 | 0.768136 |
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Target: 5'- cGGGCCgacgugggGAUCGUCcucgaacacgaugcaGGcGCCGccGGCCAa -3' miRNA: 3'- -CCUGGa-------CUAGUAG---------------UU-CGGCuuCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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