Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22337 | 3' | -58.7 | NC_005045.1 | + | 25010 | 0.66 | 0.467313 |
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Target: 5'- -uCUGGCGCucggacuccuCGGCACUCacguugaaguGACGGC-CCa -3' miRNA: 3'- guGACCGUG----------GCCGUGAG----------CUGCUGcGG- -5' |
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| 22337 | 3' | -58.7 | NC_005045.1 | + | 8622 | 0.66 | 0.467313 |
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Target: 5'- cCACgccggGGCcUCGGUGCUgGugGcaGCGCCu -3' miRNA: 3'- -GUGa----CCGuGGCCGUGAgCugC--UGCGG- -5' |
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| 22337 | 3' | -58.7 | NC_005045.1 | + | 22442 | 0.66 | 0.467313 |
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Target: 5'- aGCuUGGCgaagucGCCGauGUAgucCUCGACGugGCCg -3' miRNA: 3'- gUG-ACCG------UGGC--CGU---GAGCUGCugCGG- -5' |
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| 22337 | 3' | -58.7 | NC_005045.1 | + | 39399 | 0.66 | 0.47729 |
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Target: 5'- uGCUGGaCACCaGC-CUCGcCGACGa- -3' miRNA: 3'- gUGACC-GUGGcCGuGAGCuGCUGCgg -5' |
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| 22337 | 3' | -58.7 | NC_005045.1 | + | 8783 | 0.66 | 0.487371 |
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Target: 5'- aGCc-GCGCCuGUACUCGGCGGC-CCu -3' miRNA: 3'- gUGacCGUGGcCGUGAGCUGCUGcGG- -5' |
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| 22337 | 3' | -58.7 | NC_005045.1 | + | 26430 | 0.66 | 0.487371 |
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Target: 5'- uGCUGGCGuuguCCGGCGuCaCGuaaGugGCCg -3' miRNA: 3'- gUGACCGU----GGCCGU-GaGCug-CugCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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