miRNA: 3'- -CUGUUAUUGCCGGUCUGugUCUGGCG- -5'
miRNA: 3'- -CUGUUA-UUGCCGGUCUgU-GUC-UGG---CG- -5'
miRNA: 3'- cUGUUaUUGcCgGUCUGUGUCUGGCg -5'
miRNA: 3'- cUGuUAUUGCCGG-----UCUGUG---UCUGG---CG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22372 | 5' | -51.3 | NC_005045.1 | + | 37355 | 0.95 | 0.017904 |
|
Target: 5'- gGACAAUAACGGCCAGACcaAGACCGCc -3' miRNA: 3'- -CUGUUAUUGCCGGUCUGugUCUGGCG- -5' |
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| 22372 | 5' | -51.3 | NC_005045.1 | + | 37989 | 0.71 | 0.587022 |
|
Target: 5'- aGACGAUcGGCGGCCAGAgGgCAGcGCCuggGCg -3' miRNA: 3'- -CUGUUA-UUGCCGGUCUgU-GUC-UGG---CG- -5' |
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| 22372 | 5' | -51.3 | NC_005045.1 | + | 41258 | 0.67 | 0.799162 |
|
Target: 5'- aACGAcAGCaG-CAGGCACAGGCCGg -3' miRNA: 3'- cUGUUaUUGcCgGUCUGUGUCUGGCg -5' |
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| 22372 | 5' | -51.3 | NC_005045.1 | + | 41301 | 0.68 | 0.724373 |
|
Target: 5'- cGCuGUAGCGGCCuaucaGGACGCccuGGACCuauGCa -3' miRNA: 3'- cUGuUAUUGCCGG-----UCUGUG---UCUGG---CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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