Results 21 - 36 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22399 | 5' | -61 | NC_005056.1 | + | 4033 | 0.68 | 0.208889 |
Target: 5'- gGGCGUcaccgccaccGCCGGUGGCuuugCugCGGCGc- -3' miRNA: 3'- -UCGCG----------UGGCCGCCGua--GugGUCGCag -5' |
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22399 | 5' | -61 | NC_005056.1 | + | 8532 | 0.68 | 0.21455 |
Target: 5'- --aGCACCGuGaCGGCAUCAgUCAGCGcCc -3' miRNA: 3'- ucgCGUGGC-C-GCCGUAGU-GGUCGCaG- -5' |
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22399 | 5' | -61 | NC_005056.1 | + | 3976 | 0.67 | 0.218591 |
Target: 5'- aAGC-CACCGGCGGUggcgugacgcccugAugacgaacugcUgACCGGCGUCa -3' miRNA: 3'- -UCGcGUGGCCGCCG--------------U-----------AgUGGUCGCAG- -5' |
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22399 | 5' | -61 | NC_005056.1 | + | 8293 | 0.67 | 0.226268 |
Target: 5'- uGGUGCaACCGGCGcacaGCAUCaggcagGCCAGCa-- -3' miRNA: 3'- -UCGCG-UGGCCGC----CGUAG------UGGUCGcag -5' |
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22399 | 5' | -61 | NC_005056.1 | + | 6819 | 0.67 | 0.244858 |
Target: 5'- gGGCGCAuguugcUCGGUGGUuuugucucgaugGUUGCCGGUGUUg -3' miRNA: 3'- -UCGCGU------GGCCGCCG------------UAGUGGUCGCAG- -5' |
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22399 | 5' | -61 | NC_005056.1 | + | 18585 | 0.67 | 0.246785 |
Target: 5'- gGGCGCugCcgguguggggauugcGGCGGCGggaacgaUgGCCGGUGUUa -3' miRNA: 3'- -UCGCGugG---------------CCGCCGU-------AgUGGUCGCAG- -5' |
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22399 | 5' | -61 | NC_005056.1 | + | 10706 | 0.67 | 0.25133 |
Target: 5'- aGGUcauUACCGGCGGCAcagGCCAGCaUCa -3' miRNA: 3'- -UCGc--GUGGCCGCCGUag-UGGUCGcAG- -5' |
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22399 | 5' | -61 | NC_005056.1 | + | 5911 | 0.67 | 0.25133 |
Target: 5'- -cCGCGCCagGGCGGCA-CGCCGGUcaGUg -3' miRNA: 3'- ucGCGUGG--CCGCCGUaGUGGUCG--CAg -5' |
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22399 | 5' | -61 | NC_005056.1 | + | 2093 | 0.66 | 0.255281 |
Target: 5'- cAGUuCACCGGaccagaacggauaGGCGUCgugcGCCAGCGUg -3' miRNA: 3'- -UCGcGUGGCCg------------CCGUAG----UGGUCGCAg -5' |
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22399 | 5' | -61 | NC_005056.1 | + | 5942 | 0.66 | 0.264697 |
Target: 5'- gGGCGCauucgguuguuaACUGGCuuucuuuuucgGGCGUCcacguuuuGCCGGUGUCa -3' miRNA: 3'- -UCGCG------------UGGCCG-----------CCGUAG--------UGGUCGCAG- -5' |
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22399 | 5' | -61 | NC_005056.1 | + | 8260 | 0.66 | 0.271595 |
Target: 5'- cAGCGC-CCGGCcuGCAcagaCGCCAGUGa- -3' miRNA: 3'- -UCGCGuGGCCGc-CGUa---GUGGUCGCag -5' |
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22399 | 5' | -61 | NC_005056.1 | + | 4655 | 0.66 | 0.278635 |
Target: 5'- cGCGCGCCGgggcuucauuGCGGUAuuUCugCAGCc-- -3' miRNA: 3'- uCGCGUGGC----------CGCCGU--AGugGUCGcag -5' |
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22399 | 5' | -61 | NC_005056.1 | + | 4315 | 0.66 | 0.285821 |
Target: 5'- -cCGaCACCGcCGGUggCACCgAGCGUCa -3' miRNA: 3'- ucGC-GUGGCcGCCGuaGUGG-UCGCAG- -5' |
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22399 | 5' | -61 | NC_005056.1 | + | 1159 | 0.66 | 0.288736 |
Target: 5'- cGGgGCGCUGGCGGuCAUuuuuuuugccgcaggUugCAGUGUUu -3' miRNA: 3'- -UCgCGUGGCCGCC-GUA---------------GugGUCGCAG- -5' |
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22399 | 5' | -61 | NC_005056.1 | + | 1759 | 0.66 | 0.293152 |
Target: 5'- cAGCGCcggauGCUGGUacuGGCAcCGCCGGUGg- -3' miRNA: 3'- -UCGCG-----UGGCCG---CCGUaGUGGUCGCag -5' |
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22399 | 5' | -61 | NC_005056.1 | + | 26247 | 0.66 | 0.293152 |
Target: 5'- cGCagGCACCGGUaaGGCGUuUACCGGUGc- -3' miRNA: 3'- uCG--CGUGGCCG--CCGUA-GUGGUCGCag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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