Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22430 | 5' | -53.3 | NC_005056.1 | + | 28170 | 0.66 | 0.730139 |
Target: 5'- ---uGCugCGGCGugcucagaAGGCGUGGGaGCCg -3' miRNA: 3'- gcacUGugGCCGU--------UUUGCACCUgCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 6360 | 0.66 | 0.730139 |
Target: 5'- gCGU--UACC-GCG--GCGUGGAUGCCa -3' miRNA: 3'- -GCAcuGUGGcCGUuuUGCACCUGCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 5428 | 0.66 | 0.71909 |
Target: 5'- --gGAUACCGGCGAuauugccgguGCGcUGGAgauUGCCc -3' miRNA: 3'- gcaCUGUGGCCGUUu---------UGC-ACCU---GCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 20076 | 0.66 | 0.71909 |
Target: 5'- --aGGCACUGGCugacGCGUuGAUGCUg -3' miRNA: 3'- gcaCUGUGGCCGuuu-UGCAcCUGCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 16573 | 0.66 | 0.717979 |
Target: 5'- gCGcUGAUGCCGGUAAcaccguucaccccGACGuUGGACaGCg -3' miRNA: 3'- -GC-ACUGUGGCCGUU-------------UUGC-ACCUG-CGg -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 5982 | 0.66 | 0.707947 |
Target: 5'- gGUGAUucacccucacugACCGGCGugccgcccuGGCGcgGGGCGCa -3' miRNA: 3'- gCACUG------------UGGCCGUu--------UUGCa-CCUGCGg -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 9886 | 0.66 | 0.707947 |
Target: 5'- cCGUGAcCACCGGCAc--CGUGG-CaGUa -3' miRNA: 3'- -GCACU-GUGGCCGUuuuGCACCuG-CGg -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 4060 | 0.66 | 0.685433 |
Target: 5'- -cUGACGCCGGUcagcAGuuCGUcaucaGGGCGUCa -3' miRNA: 3'- gcACUGUGGCCG----UUuuGCA-----CCUGCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 19792 | 0.66 | 0.685433 |
Target: 5'- --cGAUuagcugGCCGGUuguugcCGUGGugGCCg -3' miRNA: 3'- gcaCUG------UGGCCGuuuu--GCACCugCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 8118 | 0.66 | 0.685433 |
Target: 5'- gGUGGCACUGcGUcaGGAAC-UGGAgaaCGCCg -3' miRNA: 3'- gCACUGUGGC-CG--UUUUGcACCU---GCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 26288 | 0.66 | 0.674087 |
Target: 5'- gGUGcCugCGGCAAaugcuGACGgagGGAcaaaaCGCCc -3' miRNA: 3'- gCACuGugGCCGUU-----UUGCa--CCU-----GCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 11560 | 0.67 | 0.628393 |
Target: 5'- --cGGCGcaauCCGGCGuGugGUGGA-GCCg -3' miRNA: 3'- gcaCUGU----GGCCGUuUugCACCUgCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 28909 | 0.68 | 0.594119 |
Target: 5'- cCGUG-CACa-GCAAAACG-GGugGCUu -3' miRNA: 3'- -GCACuGUGgcCGUUUUGCaCCugCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 5683 | 0.68 | 0.594119 |
Target: 5'- gCGUGACGCUGGUGAugcCGccGGuGCGCUg -3' miRNA: 3'- -GCACUGUGGCCGUUuu-GCa-CC-UGCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 4975 | 0.68 | 0.59298 |
Target: 5'- uCGUGAugagcaucgcaucCGCCGGgAAGuACG-GGACGCg -3' miRNA: 3'- -GCACU-------------GUGGCCgUUU-UGCaCCUGCGg -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 28380 | 0.68 | 0.58275 |
Target: 5'- --aGGCGCUGGC---GCGUGGcGCGaCCg -3' miRNA: 3'- gcaCUGUGGCCGuuuUGCACC-UGC-GG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 26250 | 0.69 | 0.548947 |
Target: 5'- --aGGCACCGGUAAGGCGUuuaccGG-UGCUu -3' miRNA: 3'- gcaCUGUGGCCGUUUUGCA-----CCuGCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 1747 | 0.69 | 0.548947 |
Target: 5'- -cUGGUACUGGCAccGCcgGUGGugGCCg -3' miRNA: 3'- gcACUGUGGCCGUuuUG--CACCugCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 7094 | 0.69 | 0.504899 |
Target: 5'- aCGUGAuaguCACCGGacuGGACG-GGAaGCCg -3' miRNA: 3'- -GCACU----GUGGCCgu-UUUGCaCCUgCGG- -5' |
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22430 | 5' | -53.3 | NC_005056.1 | + | 17818 | 0.7 | 0.481327 |
Target: 5'- uGUGGCGCUGGCcgguAAgGUGGucgguuuuuuguCGCCg -3' miRNA: 3'- gCACUGUGGCCGuu--UUgCACCu-----------GCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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