Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22482 | 3' | -41.8 | NC_005066.1 | + | 152839 | 0.66 | 1 |
Target: 5'- -aCAGCACCcu-GAACGGuuucauGCCAa -3' miRNA: 3'- caGUUGUGGauuUUUGCUuuuu--CGGU- -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 55397 | 0.66 | 1 |
Target: 5'- uGUCAACAgacaUAGGAACaguAAAGGCCAu -3' miRNA: 3'- -CAGUUGUgg--AUUUUUGcu-UUUUCGGU- -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 50114 | 0.66 | 1 |
Target: 5'- -aUAGCACCUucAAAAACaucauuGAGAGCCu -3' miRNA: 3'- caGUUGUGGA--UUUUUGcu----UUUUCGGu -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 121681 | 0.66 | 1 |
Target: 5'- uUCuACGCCUGAuu-CGAGAAcaauAGCUAg -3' miRNA: 3'- cAGuUGUGGAUUuuuGCUUUU----UCGGU- -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 121957 | 0.66 | 1 |
Target: 5'- uUCAGCGCCaacacgcuuAACGAAAAGGUUu -3' miRNA: 3'- cAGUUGUGGauuu-----UUGCUUUUUCGGu -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 102903 | 0.66 | 1 |
Target: 5'- --gGACGCCguaagcaauUGGAAGCGGAcaacGAGCCAg -3' miRNA: 3'- cagUUGUGG---------AUUUUUGCUUu---UUCGGU- -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 112720 | 0.67 | 1 |
Target: 5'- aUCAACACC-AGcAACGGcacuAGCCAc -3' miRNA: 3'- cAGUUGUGGaUUuUUGCUuuu-UCGGU- -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 93216 | 0.67 | 1 |
Target: 5'- gGUCcccGCugCUgGAGAACGAAAAAcuGCCGg -3' miRNA: 3'- -CAGu--UGugGA-UUUUUGCUUUUU--CGGU- -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 68535 | 0.68 | 1 |
Target: 5'- aUCAGCA-CUAAGGGCGcgGAAGCa- -3' miRNA: 3'- cAGUUGUgGAUUUUUGCuuUUUCGgu -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 32848 | 0.68 | 1 |
Target: 5'- aUCGcCACCUAAGAAUGuu--AGCCc -3' miRNA: 3'- cAGUuGUGGAUUUUUGCuuuuUCGGu -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 92170 | 0.68 | 1 |
Target: 5'- cGUCAgacGCACCaGAAAGCGAuAAAaCCGa -3' miRNA: 3'- -CAGU---UGUGGaUUUUUGCUuUUUcGGU- -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 64370 | 0.66 | 1 |
Target: 5'- aGUUAAacCAgCUAAAGAgacaaggccacCGGAAAAGCCAa -3' miRNA: 3'- -CAGUU--GUgGAUUUUU-----------GCUUUUUCGGU- -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 142974 | 0.66 | 1 |
Target: 5'- uUCAAUGCUUAAugaacauGCGGuAAAAGCCGc -3' miRNA: 3'- cAGUUGUGGAUUuu-----UGCU-UUUUCGGU- -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 113452 | 0.67 | 1 |
Target: 5'- gGUCaAACGCUUAAaagaaaucauGAGCGAGGAAGUa- -3' miRNA: 3'- -CAG-UUGUGGAUU----------UUUGCUUUUUCGgu -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 23126 | 0.68 | 1 |
Target: 5'- --aAACGCgUAGAAuacGCaGAGAAAGCCAa -3' miRNA: 3'- cagUUGUGgAUUUU---UG-CUUUUUCGGU- -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 10297 | 0.68 | 1 |
Target: 5'- cGUUAACGCCaUGAuucaGGACGAGGgcGCUAc -3' miRNA: 3'- -CAGUUGUGG-AUU----UUUGCUUUuuCGGU- -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 119056 | 0.67 | 1 |
Target: 5'- uUCAACACUUAuu-GCGAAGAuGUUAa -3' miRNA: 3'- cAGUUGUGGAUuuuUGCUUUUuCGGU- -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 124332 | 0.67 | 1 |
Target: 5'- cGUCAuaACCUGAu--UGGAAAAGUCAu -3' miRNA: 3'- -CAGUugUGGAUUuuuGCUUUUUCGGU- -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 11353 | 0.69 | 0.999999 |
Target: 5'- -aCAACACCgacaauAGCGGAcagcAAGCCAa -3' miRNA: 3'- caGUUGUGGauuu--UUGCUUu---UUCGGU- -5' |
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22482 | 3' | -41.8 | NC_005066.1 | + | 45272 | 0.69 | 0.999999 |
Target: 5'- uGUCAACAaCUAAAAACaaAAAGGGCUAu -3' miRNA: 3'- -CAGUUGUgGAUUUUUGc-UUUUUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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