Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22484 | 3' | -46.3 | NC_005066.1 | + | 163366 | 0.66 | 0.999986 |
Target: 5'- uUUUGAGGAAGUAGucACGGGcuuuuuugcgcguUCACCGc -3' miRNA: 3'- cGAGCUUCUUCGUUu-UGUCU-------------AGUGGC- -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 116389 | 0.66 | 0.999964 |
Target: 5'- -aUCGuc--AGCAuuaAAGCAGAUCACCu -3' miRNA: 3'- cgAGCuucuUCGU---UUUGUCUAGUGGc -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 154121 | 0.66 | 0.999951 |
Target: 5'- --aCGAGGAuuccGGCAGAAC-GAUCACg- -3' miRNA: 3'- cgaGCUUCU----UCGUUUUGuCUAGUGgc -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 59325 | 0.66 | 0.999951 |
Target: 5'- cGCa-GAAGAAGUAAAACGGu---CCGg -3' miRNA: 3'- -CGagCUUCUUCGUUUUGUCuaguGGC- -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 105263 | 0.66 | 0.999951 |
Target: 5'- aUUUGAAGAAGUuguuAAGCAGAUgCACa- -3' miRNA: 3'- cGAGCUUCUUCGu---UUUGUCUA-GUGgc -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 50817 | 0.66 | 0.999951 |
Target: 5'- cGCUCGAAcuugauGAAGCAuacAAACaAGGUCGagaCGa -3' miRNA: 3'- -CGAGCUU------CUUCGU---UUUG-UCUAGUg--GC- -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 92577 | 0.67 | 0.999914 |
Target: 5'- aGUUCGAcGGAuuC-AAACAGGUCAUCGa -3' miRNA: 3'- -CGAGCU-UCUucGuUUUGUCUAGUGGC- -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 53062 | 0.67 | 0.999887 |
Target: 5'- cGCgcgUGAAGAGGCAaagaaAAACGGuauGCCGg -3' miRNA: 3'- -CGa--GCUUCUUCGU-----UUUGUCuagUGGC- -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 27655 | 0.67 | 0.999874 |
Target: 5'- uGCUgGAAGAAGCAGAguuugaaACuGAccucgugccguauuUCGCCa -3' miRNA: 3'- -CGAgCUUCUUCGUUU-------UGuCU--------------AGUGGc -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 142168 | 0.67 | 0.999853 |
Target: 5'- uCUCGGAGAAGUAuacCAGAcuuuuaaugUCAUCGu -3' miRNA: 3'- cGAGCUUCUUCGUuuuGUCU---------AGUGGC- -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 109626 | 0.68 | 0.99981 |
Target: 5'- aCUCcaGAGGAAGCGAAgagaaaugauACucuguuugaagGGAUCACCGa -3' miRNA: 3'- cGAG--CUUCUUCGUUU----------UG-----------UCUAGUGGC- -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 141656 | 0.68 | 0.999756 |
Target: 5'- --aCGGcaGGAAGCGAAuCAGGgcuUCACCGu -3' miRNA: 3'- cgaGCU--UCUUCGUUUuGUCU---AGUGGC- -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 98044 | 0.68 | 0.999756 |
Target: 5'- aGCaaGAcGGGAGCAGGu--GAUCGCCGg -3' miRNA: 3'- -CGagCU-UCUUCGUUUuguCUAGUGGC- -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 130775 | 0.68 | 0.999689 |
Target: 5'- cGCUaGAGGAAgGUAAGACAGuaacauucuAUUACCGa -3' miRNA: 3'- -CGAgCUUCUU-CGUUUUGUC---------UAGUGGC- -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 7311 | 0.68 | 0.999597 |
Target: 5'- cGCUCGGaucaacuGGucGUAAcugguACAGAUCACCc -3' miRNA: 3'- -CGAGCU-------UCuuCGUUu----UGUCUAGUGGc -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 90379 | 0.69 | 0.999382 |
Target: 5'- aGCgUCGAGauGGUAAAGCA-AUCACCGa -3' miRNA: 3'- -CG-AGCUUcuUCGUUUUGUcUAGUGGC- -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 124753 | 0.69 | 0.999382 |
Target: 5'- aUUCGAAGAAcUAAAACAGAUUGuuGa -3' miRNA: 3'- cGAGCUUCUUcGUUUUGUCUAGUggC- -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 129724 | 0.69 | 0.998563 |
Target: 5'- aUUCGAGGAAGCAuu-CAGGUCuauucuucgcaauGCCu -3' miRNA: 3'- cGAGCUUCUUCGUuuuGUCUAG-------------UGGc -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 161490 | 0.69 | 0.998506 |
Target: 5'- -aUUGAGGAaucccgcguuccucAGCAGAAUAGAUCGCa- -3' miRNA: 3'- cgAGCUUCU--------------UCGUUUUGUCUAGUGgc -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 113426 | 0.74 | 0.973571 |
Target: 5'- --gCGAGGAAGUAuucgaAAACaAGAUCACCGc -3' miRNA: 3'- cgaGCUUCUUCGU-----UUUG-UCUAGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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