miRNA: 3'- -CGAGCU---UCuUCGUUUUGuCUAGUGGc -5'
miRNA: 3'- -CGagCU----------UCUUCGUUUUGUCUAGUGGc -5'
miRNA: 3'- cgagCUUCU---UCGUUUUGUCUAGUGGC- -5'
miRNA: 3'- -CGAg--CUUCUUCGUUUUGuCUAGUGgC- -5'
miRNA: 3'- -CGAGCUUCUUCGUUUUGUCUAGUGGC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22484 | 3' | -46.3 | NC_005066.1 | + | 80597 | 0.74 | 0.967437 |
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Target: 5'- uGCUCGGaucGGcAGCGGGGC-GAUCACCa -3' miRNA: 3'- -CGAGCU---UCuUCGUUUUGuCUAGUGGc -5' |
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| 22484 | 3' | -46.3 | NC_005066.1 | + | 74700 | 0.75 | 0.947859 |
|
Target: 5'- gGCaaGAgacuauuaccGGAGGCAGAAUGGAUCACCa -3' miRNA: 3'- -CGagCU----------UCUUCGUUUUGUCUAGUGGc -5' |
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| 22484 | 3' | -46.3 | NC_005066.1 | + | 47522 | 0.79 | 0.823504 |
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Target: 5'- ---aGAAGAaaaGGCAAAACGGAUCAUCGa -3' miRNA: 3'- cgagCUUCU---UCGUUUUGUCUAGUGGC- -5' |
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| 22484 | 3' | -46.3 | NC_005066.1 | + | 61330 | 0.8 | 0.785817 |
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Target: 5'- cGCUaaaGAGGAAGUAGAACuGAUCACgGa -3' miRNA: 3'- -CGAg--CUUCUUCGUUUUGuCUAGUGgC- -5' |
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| 22484 | 3' | -46.3 | NC_005066.1 | + | 92348 | 1.12 | 0.01861 |
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Target: 5'- uGCUCGAAGAAGCAAAACAGAUCACCGu -3' miRNA: 3'- -CGAGCUUCUUCGUUUUGUCUAGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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