Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22484 | 3' | -46.3 | NC_005066.1 | + | 141656 | 0.68 | 0.999756 |
Target: 5'- --aCGGcaGGAAGCGAAuCAGGgcuUCACCGu -3' miRNA: 3'- cgaGCU--UCUUCGUUUuGUCU---AGUGGC- -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 142168 | 0.67 | 0.999853 |
Target: 5'- uCUCGGAGAAGUAuacCAGAcuuuuaaugUCAUCGu -3' miRNA: 3'- cGAGCUUCUUCGUuuuGUCU---------AGUGGC- -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 154121 | 0.66 | 0.999951 |
Target: 5'- --aCGAGGAuuccGGCAGAAC-GAUCACg- -3' miRNA: 3'- cgaGCUUCU----UCGUUUUGuCUAGUGgc -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 161490 | 0.69 | 0.998506 |
Target: 5'- -aUUGAGGAaucccgcguuccucAGCAGAAUAGAUCGCa- -3' miRNA: 3'- cgAGCUUCU--------------UCGUUUUGUCUAGUGgc -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 163366 | 0.66 | 0.999986 |
Target: 5'- uUUUGAGGAAGUAGucACGGGcuuuuuugcgcguUCACCGc -3' miRNA: 3'- cGAGCUUCUUCGUUu-UGUCU-------------AGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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