Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22484 | 3' | -46.3 | NC_005066.1 | + | 105263 | 0.66 | 0.999951 |
Target: 5'- aUUUGAAGAAGUuguuAAGCAGAUgCACa- -3' miRNA: 3'- cGAGCUUCUUCGu---UUUGUCUA-GUGgc -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 50817 | 0.66 | 0.999951 |
Target: 5'- cGCUCGAAcuugauGAAGCAuacAAACaAGGUCGagaCGa -3' miRNA: 3'- -CGAGCUU------CUUCGU---UUUG-UCUAGUg--GC- -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 154121 | 0.66 | 0.999951 |
Target: 5'- --aCGAGGAuuccGGCAGAAC-GAUCACg- -3' miRNA: 3'- cgaGCUUCU----UCGUUUUGuCUAGUGgc -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 116389 | 0.66 | 0.999964 |
Target: 5'- -aUCGuc--AGCAuuaAAGCAGAUCACCu -3' miRNA: 3'- cgAGCuucuUCGU---UUUGUCUAGUGGc -5' |
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22484 | 3' | -46.3 | NC_005066.1 | + | 163366 | 0.66 | 0.999986 |
Target: 5'- uUUUGAGGAAGUAGucACGGGcuuuuuugcgcguUCACCGc -3' miRNA: 3'- cGAGCUUCUUCGUUu-UGUCU-------------AGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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