Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22556 | 5' | -56.3 | NC_005091.1 | + | 40782 | 0.66 | 0.693389 |
Target: 5'- aUCGCAuGGCUGGcggauuGCCAGCCGcUGCu -3' miRNA: 3'- aGGCGUcUUGGCCu-----CGGUUGGUcACG- -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 36487 | 0.66 | 0.682616 |
Target: 5'- gCUGCGGcGGCCGGAucgaaGCgGAUCAGcGCa -3' miRNA: 3'- aGGCGUC-UUGGCCU-----CGgUUGGUCaCG- -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 41010 | 0.66 | 0.682616 |
Target: 5'- -aCGgAGAcgccgGCCGGGGCCGACgAGg-- -3' miRNA: 3'- agGCgUCU-----UGGCCUCGGUUGgUCacg -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 18383 | 0.66 | 0.671797 |
Target: 5'- -gCGCAGAACguCGGAaacauGCgAGCCgugGGUGCg -3' miRNA: 3'- agGCGUCUUG--GCCU-----CGgUUGG---UCACG- -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 24943 | 0.66 | 0.671797 |
Target: 5'- cUCGUAGAcgauucggccGCCGcaguGCCGACCAGUcucGCa -3' miRNA: 3'- aGGCGUCU----------UGGCcu--CGGUUGGUCA---CG- -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 56128 | 0.66 | 0.66094 |
Target: 5'- uUCCGCAaggcuagcgucGAACuCGGGcuGCCGAUucgCAGUGUg -3' miRNA: 3'- -AGGCGU-----------CUUG-GCCU--CGGUUG---GUCACG- -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 32198 | 0.66 | 0.650057 |
Target: 5'- cUCCaGCAGcAAcCCGGAGUCGgcgcggacccgGCCGGcgGCa -3' miRNA: 3'- -AGG-CGUC-UU-GGCCUCGGU-----------UGGUCa-CG- -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 56102 | 0.66 | 0.639158 |
Target: 5'- uUCCaGCGcGGGCgGGAgGUCGGCCGGcGCg -3' miRNA: 3'- -AGG-CGU-CUUGgCCU-CGGUUGGUCaCG- -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 50770 | 0.66 | 0.639158 |
Target: 5'- gCCGCAGu-UCGcGGGCCAGCUug-GCa -3' miRNA: 3'- aGGCGUCuuGGC-CUCGGUUGGucaCG- -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 15689 | 0.67 | 0.632614 |
Target: 5'- cUCCGCuu-GCCGGuGCUGagauuuuccucggguACCAGUGg -3' miRNA: 3'- -AGGCGucuUGGCCuCGGU---------------UGGUCACg -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 28690 | 0.67 | 0.61735 |
Target: 5'- uUCUGCGGGcgcacgacacacGCCGGGGCCugcgcgcauGCCAGc-- -3' miRNA: 3'- -AGGCGUCU------------UGGCCUCGGu--------UGGUCacg -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 43979 | 0.67 | 0.606461 |
Target: 5'- -gCGCGGAAUCGGGGUgAGCUGGg-- -3' miRNA: 3'- agGCGUCUUGGCCUCGgUUGGUCacg -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 12349 | 0.67 | 0.595593 |
Target: 5'- --aGCGGcuauacaaaaaGACCGcgacGAGCgCGGCCAGUGCg -3' miRNA: 3'- aggCGUC-----------UUGGC----CUCG-GUUGGUCACG- -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 12197 | 0.67 | 0.594508 |
Target: 5'- gUCCGgcauCAGGACCcacacauGGAGCaggguauCCGGUGCg -3' miRNA: 3'- -AGGC----GUCUUGG-------CCUCGguu----GGUCACG- -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 23955 | 0.67 | 0.592338 |
Target: 5'- gCCGCAGAuGCCGG-GCCAguggcugaaucguuGCCGacGCg -3' miRNA: 3'- aGGCGUCU-UGGCCuCGGU--------------UGGUcaCG- -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 33419 | 0.67 | 0.584755 |
Target: 5'- -aCGCAGcAACCGGcauuGCCGAagacCCGGcUGCu -3' miRNA: 3'- agGCGUC-UUGGCCu---CGGUU----GGUC-ACG- -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 48782 | 0.67 | 0.584755 |
Target: 5'- -gUGCAGuGCCGcauuGGCCGGCC-GUGCg -3' miRNA: 3'- agGCGUCuUGGCc---UCGGUUGGuCACG- -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 49954 | 0.68 | 0.55678 |
Target: 5'- aCCGCAgGGGCCGacGAuGCCAACUgauaccguugcccugAGUGCc -3' miRNA: 3'- aGGCGU-CUUGGC--CU-CGGUUGG---------------UCACG- -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 58030 | 0.68 | 0.541873 |
Target: 5'- aUCgCGCuGAACgCGGAGaauCGACCGG-GCa -3' miRNA: 3'- -AG-GCGuCUUG-GCCUCg--GUUGGUCaCG- -5' |
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22556 | 5' | -56.3 | NC_005091.1 | + | 637 | 0.68 | 0.541873 |
Target: 5'- aUCgCGCuGAACgCGGAGaauCGACCGG-GCa -3' miRNA: 3'- -AG-GCGuCUUG-GCCUCg--GUUGGUCaCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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