Results 1 - 20 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22564 | 5' | -57.6 | NC_005091.1 | + | 44765 | 0.66 | 0.595593 |
Target: 5'- uUGCCGGccGCcGUGgucguugccuuCGUGCCUUcGGCCg -3' miRNA: 3'- -ACGGCUcaCGuUAC-----------GCGCGGAA-CCGG- -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 26414 | 0.66 | 0.583674 |
Target: 5'- aGCCGcAGccUGCGuugccUGCGCcuguugauucaucGCCUUGGCg -3' miRNA: 3'- aCGGC-UC--ACGUu----ACGCG-------------CGGAACCGg -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 33572 | 0.66 | 0.578271 |
Target: 5'- aUGCCggccucucgaccuauGAGcGCGAaaucGCGCGCCUcGGCg -3' miRNA: 3'- -ACGG---------------CUCaCGUUa---CGCGCGGAaCCGg -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 18360 | 0.66 | 0.573957 |
Target: 5'- aGCCGugGGUGCGAUGUauaGCGUCacaacGGCg -3' miRNA: 3'- aCGGC--UCACGUUACG---CGCGGaa---CCGg -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 25870 | 0.66 | 0.573957 |
Target: 5'- aUGCCGAG-GCGuucgGCuuGCGCCUgcacguauUGGUa -3' miRNA: 3'- -ACGGCUCaCGUua--CG--CGCGGA--------ACCGg -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 6441 | 0.66 | 0.573957 |
Target: 5'- -cCCGAGgGCAgcGUGCGCGCUgc-GCUg -3' miRNA: 3'- acGGCUCaCGU--UACGCGCGGaacCGG- -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 19465 | 0.66 | 0.563206 |
Target: 5'- cGCCGAacacgacgGCcuGcgGCGUGCCcuccgUGGCCu -3' miRNA: 3'- aCGGCUca------CG--UuaCGCGCGGa----ACCGG- -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 4224 | 0.66 | 0.562133 |
Target: 5'- gGCUGuggcagaggaugaAGUGCg--GCGUGCCg-GGCCa -3' miRNA: 3'- aCGGC-------------UCACGuuaCGCGCGGaaCCGG- -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 29078 | 0.67 | 0.541873 |
Target: 5'- -aCCGAGUGCAGUucgGCGCggagacGCCUgccccuGCCg -3' miRNA: 3'- acGGCUCACGUUA---CGCG------CGGAac----CGG- -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 28710 | 0.67 | 0.541873 |
Target: 5'- cGCCGGG-GCc-UGCGCGCau--GCCa -3' miRNA: 3'- aCGGCUCaCGuuACGCGCGgaacCGG- -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 37128 | 0.67 | 0.541873 |
Target: 5'- gGCCcGGcGCAGUGCGgGaagaCUgaUGGCCu -3' miRNA: 3'- aCGGcUCaCGUUACGCgCg---GA--ACCGG- -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 44191 | 0.67 | 0.531307 |
Target: 5'- cGCCGugcgcgacAGUGCcguAUGCgGCGUCUUcGCCu -3' miRNA: 3'- aCGGC--------UCACGu--UACG-CGCGGAAcCGG- -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 36516 | 0.67 | 0.531307 |
Target: 5'- cGCCGAuaccGUGCGcAUGgaaGCGCCgugcugcggcGGCCg -3' miRNA: 3'- aCGGCU----CACGU-UACg--CGCGGaa--------CCGG- -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 9271 | 0.67 | 0.531307 |
Target: 5'- cGCCGGcUGCAcgGCGaCGCa--GGCUc -3' miRNA: 3'- aCGGCUcACGUuaCGC-GCGgaaCCGG- -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 2882 | 0.67 | 0.530255 |
Target: 5'- gGCCGuuUGCGugGUGUGCGaauauguCCUUgcGGCCg -3' miRNA: 3'- aCGGCucACGU--UACGCGC-------GGAA--CCGG- -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 47883 | 0.67 | 0.520817 |
Target: 5'- cGCCGGGUGCGGUuuGUaGCCg-GGUCc -3' miRNA: 3'- aCGGCUCACGUUAcgCG-CGGaaCCGG- -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 50051 | 0.67 | 0.510408 |
Target: 5'- -aUCGAGcGCAAcGCGUGCC--GGCCu -3' miRNA: 3'- acGGCUCaCGUUaCGCGCGGaaCCGG- -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 33646 | 0.67 | 0.500087 |
Target: 5'- aUG-CGAGUGgAucgGCGCGUCgcaaGGCCa -3' miRNA: 3'- -ACgGCUCACgUua-CGCGCGGaa--CCGG- -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 20000 | 0.67 | 0.500087 |
Target: 5'- cUGCCGuGggcgGCGAUugguucgaGUGCGCCgaGGCg -3' miRNA: 3'- -ACGGCuCa---CGUUA--------CGCGCGGaaCCGg -5' |
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22564 | 5' | -57.6 | NC_005091.1 | + | 18310 | 0.67 | 0.500087 |
Target: 5'- cUGCCGAccucGCGAUGCGCGU---GGCg -3' miRNA: 3'- -ACGGCUca--CGUUACGCGCGgaaCCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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