Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22572 | 5' | -51 | NC_005091.1 | + | 36536 | 0.66 | 0.91809 |
Target: 5'- uGCGGcgACGCUGuAUCGUGC-GCcGAUa -3' miRNA: 3'- -CGUUugUGCGAC-UAGCACGuCGuCUG- -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 11989 | 0.66 | 0.91809 |
Target: 5'- --cGACGCGCagggGAaCGUGCAGCAc-- -3' miRNA: 3'- cguUUGUGCGa---CUaGCACGUCGUcug -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 57180 | 0.66 | 0.91809 |
Target: 5'- gGCGAAgACGCUGuucaacguUCG-GCcauGGUAGGCa -3' miRNA: 3'- -CGUUUgUGCGACu-------AGCaCG---UCGUCUG- -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 36590 | 0.66 | 0.91809 |
Target: 5'- uGCAugGGgGCGCU--UCGUGCGGC-GAUg -3' miRNA: 3'- -CGU--UUgUGCGAcuAGCACGUCGuCUG- -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 12249 | 0.66 | 0.904781 |
Target: 5'- -uGAAgGCGCUGAUCccGCcGCAGuACg -3' miRNA: 3'- cgUUUgUGCGACUAGcaCGuCGUC-UG- -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 47424 | 0.66 | 0.904781 |
Target: 5'- aGCGgcAGCGCGUcuuUGAaguUCGUcaGCAGCAGGu -3' miRNA: 3'- -CGU--UUGUGCG---ACU---AGCA--CGUCGUCUg -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 51818 | 0.66 | 0.904781 |
Target: 5'- gGCuuGCGCGC-GAUUGUGUacGGCcauGGCg -3' miRNA: 3'- -CGuuUGUGCGaCUAGCACG--UCGu--CUG- -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 55101 | 0.66 | 0.897688 |
Target: 5'- aGUAAGCACGaggGGUCGU-CAGCAcggGAUa -3' miRNA: 3'- -CGUUUGUGCga-CUAGCAcGUCGU---CUG- -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 23108 | 0.66 | 0.890308 |
Target: 5'- uGCGAugGCGU----CG-GCGGCAGGCg -3' miRNA: 3'- -CGUUugUGCGacuaGCaCGUCGUCUG- -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 23488 | 0.67 | 0.882645 |
Target: 5'- gGCGAugGCGCUGcUCGgucuguucgGCGGCGcuGCg -3' miRNA: 3'- -CGUUugUGCGACuAGCa--------CGUCGUc-UG- -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 25068 | 0.67 | 0.882645 |
Target: 5'- cGCAAGCGCgGCguucaaccGAUCG-GCGGCcacggAGGCg -3' miRNA: 3'- -CGUUUGUG-CGa-------CUAGCaCGUCG-----UCUG- -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 1008 | 0.67 | 0.882645 |
Target: 5'- cGCGGGCGCGCUGcUCG-GCGuCAauacGACg -3' miRNA: 3'- -CGUUUGUGCGACuAGCaCGUcGU----CUG- -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 28780 | 0.67 | 0.874706 |
Target: 5'- cGCAGGCcccgGCGUguGUCGUGCGcccGCAGAa -3' miRNA: 3'- -CGUUUG----UGCGacUAGCACGU---CGUCUg -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 27609 | 0.67 | 0.874706 |
Target: 5'- -uGAAUACGUcGGUCGUGUgGGCGGAa -3' miRNA: 3'- cgUUUGUGCGaCUAGCACG-UCGUCUg -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 42601 | 0.67 | 0.866497 |
Target: 5'- uCGGACGCuucguuuccgauGCauUGAUCGUGUAGCAGcCu -3' miRNA: 3'- cGUUUGUG------------CG--ACUAGCACGUCGUCuG- -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 48753 | 0.67 | 0.866497 |
Target: 5'- uGCGGGCACGCcacac--GCGGCAGGCc -3' miRNA: 3'- -CGUUUGUGCGacuagcaCGUCGUCUG- -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 8680 | 0.67 | 0.866497 |
Target: 5'- -gGGGCACGCUGuUCaGUGCAcucGUAGAa -3' miRNA: 3'- cgUUUGUGCGACuAG-CACGU---CGUCUg -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 22822 | 0.68 | 0.84033 |
Target: 5'- ----cCGCGCUGA-CGgccgGCGGCGGAa -3' miRNA: 3'- cguuuGUGCGACUaGCa---CGUCGUCUg -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 53056 | 0.68 | 0.84033 |
Target: 5'- uGCGGGguuCGCUGAUCGcgGCgAGCAuGGCg -3' miRNA: 3'- -CGUUUgu-GCGACUAGCa-CG-UCGU-CUG- -5' |
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22572 | 5' | -51 | NC_005091.1 | + | 57826 | 0.68 | 0.831125 |
Target: 5'- cGCGAcgGCGCaGGUCGaGCAGCAaucGGCg -3' miRNA: 3'- -CGUUugUGCGaCUAGCaCGUCGU---CUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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