Results 1 - 20 of 42 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22580 | 5' | -59.9 | NC_005091.1 | + | 45789 | 0.66 | 0.480678 |
Target: 5'- -uAGCCGGcgcGGCG-CGCGGCcgacgcguacaGGAGC-Ca -3' miRNA: 3'- guUCGGCU---CCGCuGCGCCG-----------UCUCGuG- -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 52794 | 0.66 | 0.480678 |
Target: 5'- --cGCgGcGGCGAucaggccgcaCGCGGCAGcAGCAUc -3' miRNA: 3'- guuCGgCuCCGCU----------GCGCCGUC-UCGUG- -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 23379 | 0.66 | 0.477702 |
Target: 5'- -cAGCCGGGGCagccGACGCaGCGccgccgaacagaccGAGCAg -3' miRNA: 3'- guUCGGCUCCG----CUGCGcCGU--------------CUCGUg -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 41202 | 0.66 | 0.474736 |
Target: 5'- --cGCCGAacacaaggacgaaccGGUgcuGACGgGGCGGuGCACg -3' miRNA: 3'- guuCGGCU---------------CCG---CUGCgCCGUCuCGUG- -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 1265 | 0.66 | 0.470795 |
Target: 5'- aAGGCUGGGGCGAauacCGGCAG-GUg- -3' miRNA: 3'- gUUCGGCUCCGCUgc--GCCGUCuCGug -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 42266 | 0.66 | 0.461017 |
Target: 5'- --uGCCGGGGCGAacaaccuaCGCGGaGGAaucggaaccGCGCa -3' miRNA: 3'- guuCGGCUCCGCU--------GCGCCgUCU---------CGUG- -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 30245 | 0.66 | 0.451348 |
Target: 5'- aAGGCCGGGGCGuCGaagcCGGCGaAGCcccGCg -3' miRNA: 3'- gUUCGGCUCCGCuGC----GCCGUcUCG---UG- -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 33964 | 0.66 | 0.451348 |
Target: 5'- gAAGCCGAuggguacgccgGGCGGCGuCGGUAGcgaauucgaGGCGu -3' miRNA: 3'- gUUCGGCU-----------CCGCUGC-GCCGUC---------UCGUg -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 7834 | 0.66 | 0.441791 |
Target: 5'- aCAAGCCGAugaaGGCGAUGaccucgaagcaGGCccauGAGUACc -3' miRNA: 3'- -GUUCGGCU----CCGCUGCg----------CCGu---CUCGUG- -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 11477 | 0.66 | 0.441791 |
Target: 5'- gCAGGCCGAagcgcaGcGUGACGCGGCcGA-CGCc -3' miRNA: 3'- -GUUCGGCU------C-CGCUGCGCCGuCUcGUG- -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 50811 | 0.66 | 0.441791 |
Target: 5'- -cGGCCGucGUGcGCGCGGCuauccGGAGCGg -3' miRNA: 3'- guUCGGCucCGC-UGCGCCG-----UCUCGUg -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 34052 | 0.66 | 0.432349 |
Target: 5'- --cGCUGaAGGCGuACGuCGGCGGuGCGa -3' miRNA: 3'- guuCGGC-UCCGC-UGC-GCCGUCuCGUg -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 4053 | 0.67 | 0.423952 |
Target: 5'- cCAAGCCGgauucAGGCGGggcguuucucaccauCGUGGCGGccgcuucgagagcaAGCGCg -3' miRNA: 3'- -GUUCGGC-----UCCGCU---------------GCGCCGUC--------------UCGUG- -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 54729 | 0.67 | 0.423026 |
Target: 5'- gGGGCCG-GGCcaaaGCGGCAcGGCAUg -3' miRNA: 3'- gUUCGGCuCCGcug-CGCCGUcUCGUG- -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 12096 | 0.67 | 0.403847 |
Target: 5'- gCGAGCCGGGccugcuugaugauGCGGaaCGGCAcggcGAGCACg -3' miRNA: 3'- -GUUCGGCUC-------------CGCUgcGCCGU----CUCGUG- -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 39258 | 0.67 | 0.395797 |
Target: 5'- uCGGGCCuucacaacacGAGGCGACGaCGGCcgcaAGCAa -3' miRNA: 3'- -GUUCGG----------CUCCGCUGC-GCCGuc--UCGUg -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 48589 | 0.67 | 0.395797 |
Target: 5'- --cGgCGAGGCGcgaaugGCGGgAGGGCGCa -3' miRNA: 3'- guuCgGCUCCGCug----CGCCgUCUCGUG- -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 7471 | 0.67 | 0.395797 |
Target: 5'- gCAGGCCGAGaaGACGa---AGAGCACg -3' miRNA: 3'- -GUUCGGCUCcgCUGCgccgUCUCGUG- -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 51584 | 0.67 | 0.394909 |
Target: 5'- --cGUCGcgugacGGGCGcgcagaaGCGCGGCAcGGGCGCg -3' miRNA: 3'- guuCGGC------UCCGC-------UGCGCCGU-CUCGUG- -5' |
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22580 | 5' | -59.9 | NC_005091.1 | + | 44886 | 0.67 | 0.386975 |
Target: 5'- gCAGGCgGuacGGGCGGCGgcaaGGCGGccgaaGGCACg -3' miRNA: 3'- -GUUCGgC---UCCGCUGCg---CCGUC-----UCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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