Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22716 | 3' | -59.8 | NC_005091.1 | + | 8507 | 0.66 | 0.473956 |
Target: 5'- gGCGCACGCGUucgagCAUCcgggcUGCGGC-CCGg -3' miRNA: 3'- aUGCGUGCGCG-----GUAGc----ACGUCGcGGU- -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 33140 | 0.66 | 0.470983 |
Target: 5'- uUGCGUGCGCuggaccuucaugaaGUCGUCGUGUucgAGCGCg- -3' miRNA: 3'- -AUGCGUGCG--------------CGGUAGCACG---UCGCGgu -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 16577 | 0.66 | 0.464083 |
Target: 5'- gUGCGUACcccaCGCCGUCGcucGguGCGUCGg -3' miRNA: 3'- -AUGCGUGc---GCGGUAGCa--CguCGCGGU- -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 21193 | 0.66 | 0.454318 |
Target: 5'- aACGCucgACGCGCUcgugAUCGauucuagaauUGCAGCGCa- -3' miRNA: 3'- aUGCG---UGCGCGG----UAGC----------ACGUCGCGgu -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 45701 | 0.66 | 0.444665 |
Target: 5'- -cUGUACGCGUCGgccgCGcGCcGCGCCGg -3' miRNA: 3'- auGCGUGCGCGGUa---GCaCGuCGCGGU- -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 43549 | 0.66 | 0.444665 |
Target: 5'- --aGCcugUGCGCCAUCGcGagaAGCGCCGg -3' miRNA: 3'- augCGu--GCGCGGUAGCaCg--UCGCGGU- -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 53741 | 0.66 | 0.441792 |
Target: 5'- aGCGCaACGCGUaccCGUGCGgcgaguauuaccauGCGCCGa -3' miRNA: 3'- aUGCG-UGCGCGguaGCACGU--------------CGCGGU- -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 37158 | 0.66 | 0.436077 |
Target: 5'- cACGUACGCGCCcggcguguucagcuuUCGguacuugGCGGCgGCCu -3' miRNA: 3'- aUGCGUGCGCGGu--------------AGCa------CGUCG-CGGu -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 49363 | 0.66 | 0.435129 |
Target: 5'- gGCGCACgcccgacgaacuGCGCC-UCGUGUGGCuGCaCGa -3' miRNA: 3'- aUGCGUG------------CGCGGuAGCACGUCG-CG-GU- -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 54203 | 0.66 | 0.435129 |
Target: 5'- gAUGC-CGCGCUAU-GUGCaAGCGCa- -3' miRNA: 3'- aUGCGuGCGCGGUAgCACG-UCGCGgu -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 11419 | 0.67 | 0.416417 |
Target: 5'- gACGCACauGCGCaGUCGaacGCGGCaGCCGc -3' miRNA: 3'- aUGCGUG--CGCGgUAGCa--CGUCG-CGGU- -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 17913 | 0.67 | 0.407247 |
Target: 5'- cACGaUACGuCGCCucgGUCGUGCGGUagacgGCCGg -3' miRNA: 3'- aUGC-GUGC-GCGG---UAGCACGUCG-----CGGU- -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 47354 | 0.67 | 0.398205 |
Target: 5'- gACGCGC-UGCCGcUCGUGCGgaacgacacGCGCUAc -3' miRNA: 3'- aUGCGUGcGCGGU-AGCACGU---------CGCGGU- -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 53548 | 0.67 | 0.398205 |
Target: 5'- gAUGCACGCGCCuucuaccucGUCGcaccauUGCcAGCGCa- -3' miRNA: 3'- aUGCGUGCGCGG---------UAGC------ACG-UCGCGgu -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 52691 | 0.67 | 0.380512 |
Target: 5'- cGCGCACGCcgGCCAgugCGacgGCuuuGCGCUg -3' miRNA: 3'- aUGCGUGCG--CGGUa--GCa--CGu--CGCGGu -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 29270 | 0.67 | 0.371865 |
Target: 5'- gACaGCuucguCGCGCCAUccgucgCGUGCAGCGUUu -3' miRNA: 3'- aUG-CGu----GCGCGGUA------GCACGUCGCGGu -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 23497 | 0.67 | 0.371865 |
Target: 5'- gACGCGCGCGgCGauggcgcugcUCGgucuguucgGCGGCGCUg -3' miRNA: 3'- aUGCGUGCGCgGU----------AGCa--------CGUCGCGGu -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 10907 | 0.68 | 0.363353 |
Target: 5'- cGCGUACGgGUCGUCGcugccgagUGCAaugcGCGCCu -3' miRNA: 3'- aUGCGUGCgCGGUAGC--------ACGU----CGCGGu -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 407 | 0.68 | 0.354977 |
Target: 5'- --aGCACGCGgaCGaucUCGUGCAG-GCCAu -3' miRNA: 3'- augCGUGCGCg-GU---AGCACGUCgCGGU- -5' |
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22716 | 3' | -59.8 | NC_005091.1 | + | 57801 | 0.68 | 0.354977 |
Target: 5'- --aGCACGCGgaCGaucUCGUGCAG-GCCAu -3' miRNA: 3'- augCGUGCGCg-GU---AGCACGUCgCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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