Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22725 | 3' | -40.6 | NC_005091.1 | + | 45317 | 0.66 | 1 |
Target: 5'- cGCGCg--GcCGGu-AGACGAcAGAGCg -3' miRNA: 3'- -CGCGauaCuGCCuuUUUGCUuUUUUG- -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 33750 | 0.66 | 1 |
Target: 5'- aCGCUGUGGCuGGAAGAggcuaucGCGGc----- -3' miRNA: 3'- cGCGAUACUG-CCUUUU-------UGCUuuuuug -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 56098 | 0.66 | 1 |
Target: 5'- aGCGCg--GGCGGGAGGuCGGcc-GGCg -3' miRNA: 3'- -CGCGauaCUGCCUUUUuGCUuuuUUG- -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 27415 | 0.66 | 1 |
Target: 5'- gGCGCUccGGgGGAuc-ACGAAAGGc- -3' miRNA: 3'- -CGCGAuaCUgCCUuuuUGCUUUUUug -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 41033 | 0.66 | 1 |
Target: 5'- aGCGCUG-GGCGGGAc-GCGGu----- -3' miRNA: 3'- -CGCGAUaCUGCCUUuuUGCUuuuuug -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 6456 | 0.66 | 1 |
Target: 5'- cGCGCUGcgcUGAaGGAAGcaggcgcGACGGAuggcGAACg -3' miRNA: 3'- -CGCGAU---ACUgCCUUU-------UUGCUUu---UUUG- -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 1014 | 0.66 | 1 |
Target: 5'- cGCGCUGcucGGCGucAAuACGAcgGAACg -3' miRNA: 3'- -CGCGAUa--CUGCcuUUuUGCUuuUUUG- -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 49292 | 0.67 | 0.999999 |
Target: 5'- cGCGUgcUGACGGggGA--GAAGccGCa -3' miRNA: 3'- -CGCGauACUGCCuuUUugCUUUuuUG- -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 40981 | 0.67 | 0.999999 |
Target: 5'- aGCGCUcgaaccgGACGGcGAcAACGAcuGGGCg -3' miRNA: 3'- -CGCGAua-----CUGCC-UUuUUGCUuuUUUG- -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 51218 | 0.66 | 0.999999 |
Target: 5'- cGCGCgucgcUGugGGAAGcAACGAu----- -3' miRNA: 3'- -CGCGau---ACugCCUUU-UUGCUuuuuug -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 37123 | 0.66 | 0.999999 |
Target: 5'- gGCGCaGUG-CGGGAAGACuGAu--GGCc -3' miRNA: 3'- -CGCGaUACuGCCUUUUUG-CUuuuUUG- -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 1712 | 0.66 | 0.999999 |
Target: 5'- aGCGCgucaaaGGGuuacuugccGAAGCGAAGGAGCa -3' miRNA: 3'- -CGCGauacugCCU---------UUUUGCUUUUUUG- -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 4822 | 0.67 | 0.999999 |
Target: 5'- cGCGCUcgGcGCGGcgGcAACGGucAAGCg -3' miRNA: 3'- -CGCGAuaC-UGCCuuU-UUGCUuuUUUG- -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 35939 | 0.66 | 0.999999 |
Target: 5'- cGCGCUgcgacuugAUGACGuuGAAGCGGuccacGACa -3' miRNA: 3'- -CGCGA--------UACUGCcuUUUUGCUuuu--UUG- -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 25240 | 0.67 | 0.999998 |
Target: 5'- uCGCUAUGGCGGcgGucgguGCGuccGGGGCg -3' miRNA: 3'- cGCGAUACUGCCuuUu----UGCuu-UUUUG- -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 29361 | 0.67 | 0.999997 |
Target: 5'- aCGCUGcacgcGACGGAuggcGCGAcGAAGCu -3' miRNA: 3'- cGCGAUa----CUGCCUuuu-UGCUuUUUUG- -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 52352 | 0.67 | 0.999997 |
Target: 5'- gGCGagcagGACGGAAu--CGAGAuGACg -3' miRNA: 3'- -CGCgaua-CUGCCUUuuuGCUUUuUUG- -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 6885 | 0.67 | 0.999996 |
Target: 5'- cGCGCa---GCGGAAGcgggcgcGACGAAGAAAg -3' miRNA: 3'- -CGCGauacUGCCUUU-------UUGCUUUUUUg -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 3552 | 0.68 | 0.999989 |
Target: 5'- aCGCcGUGACGGAc--GCGAAccuGCg -3' miRNA: 3'- cGCGaUACUGCCUuuuUGCUUuuuUG- -5' |
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22725 | 3' | -40.6 | NC_005091.1 | + | 51580 | 0.68 | 0.999989 |
Target: 5'- cGUGCgucgcGUGACGGGc--GCGcAGAAGCg -3' miRNA: 3'- -CGCGa----UACUGCCUuuuUGCuUUUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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