Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22726 | 5' | -57.9 | NC_005091.1 | + | 56334 | 0.66 | 0.606678 |
Target: 5'- gGCCG-GCGUcGUGGGgauucagaugaUACGCCGCuCGg -3' miRNA: 3'- -CGGUaCGCAaCGCCC-----------GUGCGGUGuGC- -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 31725 | 0.66 | 0.595939 |
Target: 5'- aCCAUGcCGUacuggUGUGGG-ACGCCGC-CGc -3' miRNA: 3'- cGGUAC-GCA-----ACGCCCgUGCGGUGuGC- -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 33422 | 0.66 | 0.574557 |
Target: 5'- aGCaggGCGUccucGCGaGCGCGCUGCGCGa -3' miRNA: 3'- -CGguaCGCAa---CGCcCGUGCGGUGUGC- -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 9723 | 0.66 | 0.574557 |
Target: 5'- uGCCAgagGCGauucUGCGGGUuCGCaauGCGCa -3' miRNA: 3'- -CGGUa--CGCa---ACGCCCGuGCGg--UGUGc -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 19559 | 0.67 | 0.553356 |
Target: 5'- gGCCA--CGga--GGGCACGCCGCAgGc -3' miRNA: 3'- -CGGUacGCaacgCCCGUGCGGUGUgC- -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 29607 | 0.67 | 0.54704 |
Target: 5'- cGCCGaucuCGgUGCGGGCgagcgggagggccguACGCUGCGCGg -3' miRNA: 3'- -CGGUac--GCaACGCCCG---------------UGCGGUGUGC- -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 49297 | 0.67 | 0.52202 |
Target: 5'- cCCAUcGCGUgcUGaCGGGgGagaaGCCGCACGu -3' miRNA: 3'- cGGUA-CGCA--AC-GCCCgUg---CGGUGUGC- -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 26507 | 0.67 | 0.52202 |
Target: 5'- gGCCAUGgacuuCGUcaggcuggccgUGCGGGCAuuggcCGCCuGCACa -3' miRNA: 3'- -CGGUAC-----GCA-----------ACGCCCGU-----GCGG-UGUGc -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 4314 | 0.67 | 0.518923 |
Target: 5'- aUCGUGCugacguacggcacggUGaCGGGCGCGCCGC-CGg -3' miRNA: 3'- cGGUACGca-------------AC-GCCCGUGCGGUGuGC- -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 3036 | 0.67 | 0.517892 |
Target: 5'- uCCcgGCGcuuucaucguacgUGCGGGCAgCGCCcaGCGCa -3' miRNA: 3'- cGGuaCGCa------------ACGCCCGU-GCGG--UGUGc -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 31678 | 0.67 | 0.501515 |
Target: 5'- cGCCAaGCagccuucGCGGGCACGCCGg--- -3' miRNA: 3'- -CGGUaCGcaa----CGCCCGUGCGGUgugc -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 23225 | 0.67 | 0.501515 |
Target: 5'- aCCGUGcCGcUGUGGuaGCGCgGCGCGu -3' miRNA: 3'- cGGUAC-GCaACGCCcgUGCGgUGUGC- -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 48763 | 0.69 | 0.43284 |
Target: 5'- gGCCGUGCGcacGCaugacGGCAUGCCuguauCACGg -3' miRNA: 3'- -CGGUACGCaa-CGc----CCGUGCGGu----GUGC- -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 50883 | 0.69 | 0.43284 |
Target: 5'- cCCAUGCuucGCGcGGCuauaGCCACACGc -3' miRNA: 3'- cGGUACGcaaCGC-CCGug--CGGUGUGC- -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 22248 | 0.69 | 0.411491 |
Target: 5'- uGCCGaccUGCGcggaUUgcuguacgcacugcGCGGGCGCGUCACGCc -3' miRNA: 3'- -CGGU---ACGC----AA--------------CGCCCGUGCGGUGUGc -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 15715 | 0.69 | 0.396138 |
Target: 5'- uGCCGUGCccccgguuGUUGCGaucuGCGCGCCAUuCGu -3' miRNA: 3'- -CGGUACG--------CAACGCc---CGUGCGGUGuGC- -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 43875 | 0.69 | 0.387281 |
Target: 5'- aGCCAUGCucuaUGCGcucGGCGCGCUcUACGa -3' miRNA: 3'- -CGGUACGca--ACGC---CCGUGCGGuGUGC- -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 997 | 0.7 | 0.369962 |
Target: 5'- gGCC-UGCucgacGCGGGCGCGCUGCuCGg -3' miRNA: 3'- -CGGuACGcaa--CGCCCGUGCGGUGuGC- -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 23770 | 0.7 | 0.35318 |
Target: 5'- aCCAUGCGcugaaucUGCGGGC-CGCUcuccguguACGCGg -3' miRNA: 3'- cGGUACGCa------ACGCCCGuGCGG--------UGUGC- -5' |
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22726 | 5' | -57.9 | NC_005091.1 | + | 1224 | 0.7 | 0.35318 |
Target: 5'- cGCCAguuaGCGUaaUGCGccuuCACGCCACGCa -3' miRNA: 3'- -CGGUa---CGCA--ACGCcc--GUGCGGUGUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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