Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22729 | 3' | -54.3 | NC_005091.1 | + | 30796 | 0.66 | 0.807464 |
Target: 5'- cCGU-CGuCCAGUCGCGaGCGGuugaaguCGGACGu -3' miRNA: 3'- -GUAcGC-GGUCAGUGC-CGCUu------GCUUGC- -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 57425 | 0.66 | 0.797897 |
Target: 5'- gCAUGCGCgAGUCG-GGCGuuccuaucAugGGGCu -3' miRNA: 3'- -GUACGCGgUCAGUgCCGC--------UugCUUGc -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 53066 | 0.66 | 0.797897 |
Target: 5'- gAUGCGCCccguaaccguuGGUCgcgagcauuGCGGCGAcCGuuuGCGg -3' miRNA: 3'- gUACGCGG-----------UCAG---------UGCCGCUuGCu--UGC- -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 31 | 0.66 | 0.797897 |
Target: 5'- gCAUGCGCgAGUCG-GGCGuuccuaucAugGGGCu -3' miRNA: 3'- -GUACGCGgUCAGUgCCGC--------UugCUUGc -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 29464 | 0.66 | 0.797897 |
Target: 5'- -cUGUcCCGGUU-CGGCGAaccggGCGAGCGa -3' miRNA: 3'- guACGcGGUCAGuGCCGCU-----UGCUUGC- -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 7533 | 0.66 | 0.788157 |
Target: 5'- aAU-CGCCGGUCggcACGGCGGAaGGGCu -3' miRNA: 3'- gUAcGCGGUCAG---UGCCGCUUgCUUGc -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 35229 | 0.66 | 0.778255 |
Target: 5'- ---aCGCCAG-CGCGGCG-GCGAuuuucGCGa -3' miRNA: 3'- guacGCGGUCaGUGCCGCuUGCU-----UGC- -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 19399 | 0.66 | 0.797897 |
Target: 5'- gCAUGUGCgAG-CGCGGCGcuCGGGa- -3' miRNA: 3'- -GUACGCGgUCaGUGCCGCuuGCUUgc -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 52121 | 0.66 | 0.807464 |
Target: 5'- cCGU-CGCCGGcgCGgGGCGcguuGCGGACGa -3' miRNA: 3'- -GUAcGCGGUCa-GUgCCGCu---UGCUUGC- -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 17875 | 0.66 | 0.797897 |
Target: 5'- -cUGUGCgAGaUCGgcCGGCGAcaacugGCGAACGa -3' miRNA: 3'- guACGCGgUC-AGU--GCCGCU------UGCUUGC- -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 57221 | 0.67 | 0.747689 |
Target: 5'- ----aGCCAGUCggGCGGCGAgucGCGGAa- -3' miRNA: 3'- guacgCGGUCAG--UGCCGCU---UGCUUgc -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 3625 | 0.67 | 0.737251 |
Target: 5'- --cGCGUCcGUCACGGCGuGCaGGAuCGg -3' miRNA: 3'- guaCGCGGuCAGUGCCGCuUG-CUU-GC- -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 51646 | 0.67 | 0.70535 |
Target: 5'- --aGCGgcuuuCCAGuUCGCGGCcauucGGGCGAGCGa -3' miRNA: 3'- guaCGC-----GGUC-AGUGCCG-----CUUGCUUGC- -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 31542 | 0.67 | 0.747689 |
Target: 5'- --gGCGCUcGUUACaGCG-ACGAGCGu -3' miRNA: 3'- guaCGCGGuCAGUGcCGCuUGCUUGC- -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 36306 | 0.67 | 0.747689 |
Target: 5'- aAUGCGCgAgcGUCGCGGCGuGCcGACc -3' miRNA: 3'- gUACGCGgU--CAGUGCCGCuUGcUUGc -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 17286 | 0.67 | 0.756983 |
Target: 5'- gAUGCGCCcaugugaagaucaGGUCcccACGGaCGGACGAGa- -3' miRNA: 3'- gUACGCGG-------------UCAG---UGCC-GCUUGCUUgc -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 12116 | 0.67 | 0.75801 |
Target: 5'- gAUGCGgaaCGG-CACGGCGAgcACGAugaugGCGa -3' miRNA: 3'- gUACGCg--GUCaGUGCCGCU--UGCU-----UGC- -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 38264 | 0.67 | 0.75801 |
Target: 5'- cCGUGUGCCAcGgacgC-CGGaUGAACGAGCa -3' miRNA: 3'- -GUACGCGGU-Ca---GuGCC-GCUUGCUUGc -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 57895 | 0.67 | 0.747689 |
Target: 5'- -cUGCGCC-GUCGCGuGCGugaGAACc -3' miRNA: 3'- guACGCGGuCAGUGC-CGCuugCUUGc -5' |
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22729 | 3' | -54.3 | NC_005091.1 | + | 501 | 0.67 | 0.747689 |
Target: 5'- -cUGCGCC-GUCGCGuGCGugaGAACc -3' miRNA: 3'- guACGCGGuCAGUGC-CGCuugCUUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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