Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22734 | 3' | -67.2 | NC_005091.1 | + | 53820 | 0.66 | 0.231149 |
Target: 5'- aCGCCGgaauucacguuuGCCGGCUUCaauuuCCCGCGCUa -3' miRNA: 3'- -GCGGC------------CGGCUGGAGggc--GGGCGCGAc -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 1543 | 0.66 | 0.236756 |
Target: 5'- gGCCGGCCG-CCggaUCGUCCG-GCUu -3' miRNA: 3'- gCGGCCGGCuGGag-GGCGGGCgCGAc -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 51045 | 0.66 | 0.242478 |
Target: 5'- uCGCUuGCCGAUUUgCCCGUgcgcaCCGCGCUu -3' miRNA: 3'- -GCGGcCGGCUGGA-GGGCG-----GGCGCGAc -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 9288 | 0.66 | 0.242478 |
Target: 5'- cCGCUGGCCgccGACCggCUGgCCGUGCg- -3' miRNA: 3'- -GCGGCCGG---CUGGagGGCgGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 30429 | 0.66 | 0.225654 |
Target: 5'- gGCCGGCCGAUgaCCuaCGCUacgcggguCGCGUUGu -3' miRNA: 3'- gCGGCCGGCUGgaGG--GCGG--------GCGCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 26339 | 0.66 | 0.225654 |
Target: 5'- gCGcCCGGCCGcuacgauggcaGCC-CCggcgGCCCGCGCg- -3' miRNA: 3'- -GC-GGCCGGC-----------UGGaGGg---CGGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 32845 | 0.66 | 0.220269 |
Target: 5'- uCGaugaCGGCCaugaugGGCUUUUCGuCCCGCGCUGc -3' miRNA: 3'- -GCg---GCCGG------CUGGAGGGC-GGGCGCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 12104 | 0.66 | 0.225654 |
Target: 5'- -aCUGGCCGGCCaUCUgaaCGUCUGCGCg- -3' miRNA: 3'- gcGGCCGGCUGG-AGG---GCGGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 25654 | 0.67 | 0.181043 |
Target: 5'- aGCUGGCCGcgcaguUCUUCCGCCUGCacCUGc -3' miRNA: 3'- gCGGCCGGCu-----GGAGGGCGGGCGc-GAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 44529 | 0.67 | 0.204772 |
Target: 5'- gGCCGucgcuGCCGucGCCUUcgucgCCGCCCGCGUc- -3' miRNA: 3'- gCGGC-----CGGC--UGGAG-----GGCGGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 32100 | 0.67 | 0.194972 |
Target: 5'- cCGCCGGCCggguccgcgccGACUUCCgGgUUGCuGCUGg -3' miRNA: 3'- -GCGGCCGG-----------CUGGAGGgCgGGCG-CGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 53927 | 0.67 | 0.181043 |
Target: 5'- gCGCCaGCCGAaacaaugCCUGCCCGUGUg- -3' miRNA: 3'- -GCGGcCGGCUgga----GGGCGGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 3234 | 0.67 | 0.181043 |
Target: 5'- cCGCCGGCCgcucgaucgauGACUUCaa-CCCGCGCa- -3' miRNA: 3'- -GCGGCCGG-----------CUGGAGggcGGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 11439 | 0.67 | 0.181043 |
Target: 5'- aCGUCGGCCGACgCUUUCGCCuugacgaucCG-GCUGu -3' miRNA: 3'- -GCGGCCGGCUG-GAGGGCGG---------GCgCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 48809 | 0.67 | 0.204772 |
Target: 5'- aCGCaUGGCCGuAUCUCCguguCGCUCGUGCa- -3' miRNA: 3'- -GCG-GCCGGC-UGGAGG----GCGGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 55686 | 0.67 | 0.204272 |
Target: 5'- uGCCGcaucacuucGCCGAUCgccgCCUGCacgagcgcguggaCCGCGCUGa -3' miRNA: 3'- gCGGC---------CGGCUGGa---GGGCG-------------GGCGCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 33589 | 0.67 | 0.203275 |
Target: 5'- aGCCGugaugcggaucgauGCCGGCCUCUCGaCCUaugaGCGCg- -3' miRNA: 3'- gCGGC--------------CGGCUGGAGGGC-GGG----CGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 30890 | 0.67 | 0.194972 |
Target: 5'- aGCaCGuCCGACUUCaaCCGCUCGCGaCUGg -3' miRNA: 3'- gCG-GCcGGCUGGAG--GGCGGGCGC-GAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 28206 | 0.67 | 0.190228 |
Target: 5'- aCGCCGGCCGAaaUCCCGUCaCGaaCUu -3' miRNA: 3'- -GCGGCCGGCUggAGGGCGG-GCgcGAc -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 17634 | 0.67 | 0.185585 |
Target: 5'- gGuuGcGUCGACacgCUCCCGgCCGCGCa- -3' miRNA: 3'- gCggC-CGGCUG---GAGGGCgGGCGCGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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