Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22734 | 3' | -67.2 | NC_005091.1 | + | 33220 | 0.74 | 0.054889 |
Target: 5'- gGCCGGCguaaugcgcggCGACCaUCUGCCCGCGCa- -3' miRNA: 3'- gCGGCCG-----------GCUGGaGGGCGGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 9288 | 0.66 | 0.242478 |
Target: 5'- cCGCUGGCCgccGACCggCUGgCCGUGCg- -3' miRNA: 3'- -GCGGCCGG---CUGGagGGCgGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 30429 | 0.66 | 0.225654 |
Target: 5'- gGCCGGCCGAUgaCCuaCGCUacgcggguCGCGUUGu -3' miRNA: 3'- gCGGCCGGCUGgaGG--GCGG--------GCGCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 26339 | 0.66 | 0.225654 |
Target: 5'- gCGcCCGGCCGcuacgauggcaGCC-CCggcgGCCCGCGCg- -3' miRNA: 3'- -GC-GGCCGGC-----------UGGaGGg---CGGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 32845 | 0.66 | 0.220269 |
Target: 5'- uCGaugaCGGCCaugaugGGCUUUUCGuCCCGCGCUGc -3' miRNA: 3'- -GCg---GCCGG------CUGGAGGGC-GGGCGCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 44529 | 0.67 | 0.204772 |
Target: 5'- gGCCGucgcuGCCGucGCCUUcgucgCCGCCCGCGUc- -3' miRNA: 3'- gCGGC-----CGGC--UGGAG-----GGCGGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 32100 | 0.67 | 0.194972 |
Target: 5'- cCGCCGGCCggguccgcgccGACUUCCgGgUUGCuGCUGg -3' miRNA: 3'- -GCGGCCGG-----------CUGGAGGgCgGGCG-CGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 53927 | 0.67 | 0.181043 |
Target: 5'- gCGCCaGCCGAaacaaugCCUGCCCGUGUg- -3' miRNA: 3'- -GCGGcCGGCUgga----GGGCGGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 3234 | 0.67 | 0.181043 |
Target: 5'- cCGCCGGCCgcucgaucgauGACUUCaa-CCCGCGCa- -3' miRNA: 3'- -GCGGCCGG-----------CUGGAGggcGGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 11439 | 0.67 | 0.181043 |
Target: 5'- aCGUCGGCCGACgCUUUCGCCuugacgaucCG-GCUGu -3' miRNA: 3'- -GCGGCCGGCUG-GAGGGCGG---------GCgCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 25654 | 0.67 | 0.181043 |
Target: 5'- aGCUGGCCGcgcaguUCUUCCGCCUGCacCUGc -3' miRNA: 3'- gCGGCCGGCu-----GGAGGGCGGGCGc-GAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 53597 | 0.68 | 0.176599 |
Target: 5'- gCGCCGuCCGAuuccaaauagaaCCcCuuGCCCGUGCUGu -3' miRNA: 3'- -GCGGCcGGCU------------GGaGggCGGGCGCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 53423 | 0.69 | 0.14926 |
Target: 5'- uCGCUGGCCgugcauucgcccuuaGACCaUCCCGCcagaaagCCGCGCc- -3' miRNA: 3'- -GCGGCCGG---------------CUGG-AGGGCG-------GGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 55907 | 0.68 | 0.168002 |
Target: 5'- gCGCagcaGGCCGACCaaCUGCgUGCGCa- -3' miRNA: 3'- -GCGg---CCGGCUGGagGGCGgGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 56015 | 1.08 | 0.00012 |
Target: 5'- gCGCCGGCCGACCUCCCGCCCGCGCUGg -3' miRNA: 3'- -GCGGCCGGCUGGAGGGCGGGCGCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 4313 | 0.72 | 0.077511 |
Target: 5'- gCGCCGGCCGucguCUUCCUGuuuCCCG-GCUGg -3' miRNA: 3'- -GCGGCCGGCu---GGAGGGC---GGGCgCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 54989 | 0.72 | 0.088171 |
Target: 5'- uCGuCCGGCCGGgCggCCCGCcgguaucCCGUGCUGa -3' miRNA: 3'- -GC-GGCCGGCUgGa-GGGCG-------GGCGCGAC- -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 56269 | 0.71 | 0.095372 |
Target: 5'- aCGCCGGCCGAauaaucggUCUCCCGCuguaaauCCGgGCc- -3' miRNA: 3'- -GCGGCCGGCU--------GGAGGGCG-------GGCgCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 34757 | 0.71 | 0.095621 |
Target: 5'- uGUCGGCC-AUgUCCCGgCCGCGCg- -3' miRNA: 3'- gCGGCCGGcUGgAGGGCgGGCGCGac -5' |
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22734 | 3' | -67.2 | NC_005091.1 | + | 40928 | 0.7 | 0.117686 |
Target: 5'- gCGUaCGGCCGAgguugugaaCCugaacaccgcgUCCCGCCCaGCGCUGc -3' miRNA: 3'- -GCG-GCCGGCU---------GG-----------AGGGCGGG-CGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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