Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22737 | 3' | -60.5 | NC_005091.1 | + | 4301 | 0.66 | 0.477956 |
Target: 5'- aCGGCa-CgGUGACgggcgCGCCGCCgGGCa -3' miRNA: 3'- -GUCGaaGgCGCUGa----GCGGCGGgCUGa -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 18142 | 0.66 | 0.477956 |
Target: 5'- uCGGUcggCGCGAgcUUCGCgGCCCGGCg -3' miRNA: 3'- -GUCGaagGCGCU--GAGCGgCGGGCUGa -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 11310 | 0.66 | 0.474056 |
Target: 5'- gCGGCUUCaacgccuuguugaGCGGCU-GCCGCguUCGACUg -3' miRNA: 3'- -GUCGAAGg------------CGCUGAgCGGCG--GGCUGA- -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 22994 | 0.66 | 0.468237 |
Target: 5'- uGGuCUUCCGCGAC-CGaaugGUCCGGCg -3' miRNA: 3'- gUC-GAAGGCGCUGaGCgg--CGGGCUGa -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 55315 | 0.66 | 0.468237 |
Target: 5'- aCAGCUUugccgcCCGCGuGCUCGC-GCUCGAa- -3' miRNA: 3'- -GUCGAA------GGCGC-UGAGCGgCGGGCUga -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 6091 | 0.66 | 0.449109 |
Target: 5'- -cGCg-CCGCuucGGCUCGUCGCCCG-Ca -3' miRNA: 3'- guCGaaGGCG---CUGAGCGGCGGGCuGa -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 50887 | 0.66 | 0.449109 |
Target: 5'- -uGCUUCgCGCGGCUauaGCCacacGCUCGAUg -3' miRNA: 3'- guCGAAG-GCGCUGAg--CGG----CGGGCUGa -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 50420 | 0.66 | 0.449109 |
Target: 5'- aCAGCgaCCGUGAaaacUUCGCCGUUCGcACg -3' miRNA: 3'- -GUCGaaGGCGCU----GAGCGGCGGGC-UGa -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 37026 | 0.66 | 0.430422 |
Target: 5'- uCAGCagcCCGCGACUUcuCCGUgCGACg -3' miRNA: 3'- -GUCGaa-GGCGCUGAGc-GGCGgGCUGa -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 24532 | 0.66 | 0.430422 |
Target: 5'- uCGGg-UCCGCGAUgucguacaaGCCGCCgGACg -3' miRNA: 3'- -GUCgaAGGCGCUGag-------CGGCGGgCUGa -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 9282 | 0.67 | 0.42125 |
Target: 5'- -uGCUcgCCGCuGGC-CGCCGaCCGGCUg -3' miRNA: 3'- guCGAa-GGCG-CUGaGCGGCgGGCUGA- -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 468 | 0.67 | 0.412198 |
Target: 5'- uCAGCgcgCagGCGACaagcCGCCGUCCGAUg -3' miRNA: 3'- -GUCGaa-Gg-CGCUGa---GCGGCGGGCUGa -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 36882 | 0.67 | 0.403267 |
Target: 5'- uGGCcgUCCGUGaccgaguucGCUgGCCGUCCGAUc -3' miRNA: 3'- gUCGa-AGGCGC---------UGAgCGGCGGGCUGa -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 16482 | 0.67 | 0.394459 |
Target: 5'- cCAGCcggaauucUUCCGCGcguacuGCUUGCCGUcugCCGGCg -3' miRNA: 3'- -GUCG--------AAGGCGC------UGAGCGGCG---GGCUGa -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 44827 | 0.67 | 0.394459 |
Target: 5'- -uGCUUCCGCGAUU-GCCuCCgUGACUg -3' miRNA: 3'- guCGAAGGCGCUGAgCGGcGG-GCUGA- -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 53581 | 0.67 | 0.385777 |
Target: 5'- cCAGCgcaaCCcCGGCgCGCCGUCCGAUUc -3' miRNA: 3'- -GUCGaa--GGcGCUGaGCGGCGGGCUGA- -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 3343 | 0.68 | 0.372151 |
Target: 5'- -uGCUUCCgGCGugUCccuuuucgaaggcauGCCGCUCGugUc -3' miRNA: 3'- guCGAAGG-CGCugAG---------------CGGCGGGCugA- -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 17324 | 0.68 | 0.355585 |
Target: 5'- gAGaCUUCCGCGuagcGCUgGCCguucagcuucagauuGCCCGGCg -3' miRNA: 3'- gUC-GAAGGCGC----UGAgCGG---------------CGGGCUGa -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 33734 | 0.68 | 0.351525 |
Target: 5'- aGGCUaUCGCGGCUggugaagucccgaUGCCGCCgGGCc -3' miRNA: 3'- gUCGAaGGCGCUGA-------------GCGGCGGgCUGa -5' |
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22737 | 3' | -60.5 | NC_005091.1 | + | 6832 | 0.68 | 0.344301 |
Target: 5'- gGGaCUUCCGCGAC-CG-UGCCgGGCUg -3' miRNA: 3'- gUC-GAAGGCGCUGaGCgGCGGgCUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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