Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22832 | 5' | -49.9 | NC_005135.1 | + | 115728 | 0.66 | 0.997916 |
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Target: 5'- cGAACAaAUCaCCGAAUCUcGAGCCa- -3' miRNA: 3'- aCUUGUaUAG-GGUUUGGGcUUCGGcg -5' |
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| 22832 | 5' | -49.9 | NC_005135.1 | + | 55683 | 0.66 | 0.998227 |
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Target: 5'- cUGGACAaggcugaaaagguUG-CCCAgcaacaaguuaaGACugCCGAGGCCGCa -3' miRNA: 3'- -ACUUGU-------------AUaGGGU------------UUG--GGCUUCGGCG- -5' |
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| 22832 | 5' | -49.9 | NC_005135.1 | + | 11566 | 0.66 | 0.998259 |
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Target: 5'- aUGGuCAUAUCgCGAACCaucaAAGUCGCu -3' miRNA: 3'- -ACUuGUAUAGgGUUUGGgc--UUCGGCG- -5' |
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| 22832 | 5' | -49.9 | NC_005135.1 | + | 14739 | 0.66 | 0.998498 |
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Target: 5'- aUGAGCA-AUCCCAAGCCaaaugcuauuugGAAaCCGUg -3' miRNA: 3'- -ACUUGUaUAGGGUUUGGg-----------CUUcGGCG- -5' |
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| 22832 | 5' | -49.9 | NC_005135.1 | + | 100453 | 0.66 | 0.998526 |
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Target: 5'- cUGGugAUAUUCCugaagcuuacaaaAAGCCUGGAGuuGUu -3' miRNA: 3'- -ACUugUAUAGGG-------------UUUGGGCUUCggCG- -5' |
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| 22832 | 5' | -49.9 | NC_005135.1 | + | 17355 | 0.66 | 0.998554 |
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Target: 5'- cGAGC--GUCUCAGGCCCaGGAGaaGUa -3' miRNA: 3'- aCUUGuaUAGGGUUUGGG-CUUCggCG- -5' |
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| 22832 | 5' | -49.9 | NC_005135.1 | + | 75263 | 0.66 | 0.998554 |
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Target: 5'- cGGuCAUAUCCaAGACCguUGAAGUCGUc -3' miRNA: 3'- aCUuGUAUAGGgUUUGG--GCUUCGGCG- -5' |
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| 22832 | 5' | -49.9 | NC_005135.1 | + | 39186 | 0.66 | 0.998554 |
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Target: 5'- -cAGCAaauUUCCuuGCCCuugguGAAGCCGCu -3' miRNA: 3'- acUUGUau-AGGGuuUGGG-----CUUCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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