Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22832 | 5' | -49.9 | NC_005135.1 | + | 100453 | 0.66 | 0.998526 |
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Target: 5'- cUGGugAUAUUCCugaagcuuacaaaAAGCCUGGAGuuGUu -3' miRNA: 3'- -ACUugUAUAGGG-------------UUUGGGCUUCggCG- -5' |
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| 22832 | 5' | -49.9 | NC_005135.1 | + | 115728 | 0.66 | 0.997916 |
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Target: 5'- cGAACAaAUCaCCGAAUCUcGAGCCa- -3' miRNA: 3'- aCUUGUaUAG-GGUUUGGGcUUCGGcg -5' |
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| 22832 | 5' | -49.9 | NC_005135.1 | + | 127330 | 0.71 | 0.954788 |
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Target: 5'- cGAACGUcccacuUCUCAAACCC---GCCGCg -3' miRNA: 3'- aCUUGUAu-----AGGGUUUGGGcuuCGGCG- -5' |
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| 22832 | 5' | -49.9 | NC_005135.1 | + | 138271 | 0.66 | 0.997386 |
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Target: 5'- gGAAUAUgAUCCUcacccgccaaucaaGAugCCGAuGGCCGUa -3' miRNA: 3'- aCUUGUA-UAGGG--------------UUugGGCU-UCGGCG- -5' |
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| 22832 | 5' | -49.9 | NC_005135.1 | + | 147187 | 0.68 | 0.990253 |
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Target: 5'- -cGAUAUAUCCUAcACCCuuAGCUGUu -3' miRNA: 3'- acUUGUAUAGGGUuUGGGcuUCGGCG- -5' |
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| 22832 | 5' | -49.9 | NC_005135.1 | + | 157973 | 0.66 | 0.997517 |
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Target: 5'- aGAACAUcguugCUCAAGCCUGcuAAGCCa- -3' miRNA: 3'- aCUUGUAua---GGGUUUGGGC--UUCGGcg -5' |
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| 22832 | 5' | -49.9 | NC_005135.1 | + | 161559 | 0.68 | 0.991485 |
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Target: 5'- cGGAUAUGUCCaUAAGCCCGAguuuggauauGGUuucUGCg -3' miRNA: 3'- aCUUGUAUAGG-GUUUGGGCU----------UCG---GCG- -5' |
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| 22832 | 5' | -49.9 | NC_005135.1 | + | 169970 | 0.67 | 0.994457 |
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Target: 5'- aGAGCAUAU-CgAAACCgGuAAGCCGg -3' miRNA: 3'- aCUUGUAUAgGgUUUGGgC-UUCGGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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