Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22834 | 3' | -45.4 | NC_005135.1 | + | 82608 | 0.66 | 0.999994 |
Target: 5'- --cGCAAAAAUAGUaucUUCCGuugGGAGaGCu -3' miRNA: 3'- uuaUGUUUUUAUCG---GAGGCu--UCUC-CG- -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 154704 | 0.66 | 0.999991 |
Target: 5'- ---cCAAAGG-AGCC-CCGAAG-GGCu -3' miRNA: 3'- uuauGUUUUUaUCGGaGGCUUCuCCG- -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 65816 | 0.66 | 0.999991 |
Target: 5'- uAAUGCGcAGGAUGGCC----AAGAGGCa -3' miRNA: 3'- -UUAUGU-UUUUAUCGGaggcUUCUCCG- -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 43248 | 0.67 | 0.999965 |
Target: 5'- cAAUGCAuucgcgacuuAGGGUGGCgaCCGucGAGGUg -3' miRNA: 3'- -UUAUGU----------UUUUAUCGgaGGCuuCUCCG- -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 166803 | 0.67 | 0.999937 |
Target: 5'- ---cCAAAA--GGCCUCUucGGAGGCc -3' miRNA: 3'- uuauGUUUUuaUCGGAGGcuUCUCCG- -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 30227 | 0.68 | 0.999854 |
Target: 5'- uAUACGAcAAaGGCCUCacuuGGAGGCc -3' miRNA: 3'- uUAUGUUuUUaUCGGAGgcu-UCUCCG- -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 42226 | 0.68 | 0.99981 |
Target: 5'- ---cCGAA---AGcCCUCCGAAGAGGg -3' miRNA: 3'- uuauGUUUuuaUC-GGAGGCUUCUCCg -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 25909 | 0.68 | 0.999755 |
Target: 5'- ---uCGAAuaucuGCCUCCGAAaAGGCa -3' miRNA: 3'- uuauGUUUuuau-CGGAGGCUUcUCCG- -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 154874 | 0.69 | 0.999686 |
Target: 5'- uGGUACGGGucguGCUUCUcauaGAGGAGGCg -3' miRNA: 3'- -UUAUGUUUuuauCGGAGG----CUUCUCCG- -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 100794 | 0.69 | 0.999686 |
Target: 5'- cGGUGCAccGAAAUAGagaCUCgCGAAggcGAGGCu -3' miRNA: 3'- -UUAUGU--UUUUAUCg--GAG-GCUU---CUCCG- -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 11304 | 0.69 | 0.999601 |
Target: 5'- --gACAAAAAUAGUUUuuGggGAaGCa -3' miRNA: 3'- uuaUGUUUUUAUCGGAggCuuCUcCG- -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 30439 | 0.69 | 0.999497 |
Target: 5'- --aGCAAGGAagaaGGCCUCCaaguGAGGCc -3' miRNA: 3'- uuaUGUUUUUa---UCGGAGGcuu-CUCCG- -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 35084 | 0.72 | 0.994944 |
Target: 5'- uAAU-CAAAAcgGGCCUCUccGGAGGCc -3' miRNA: 3'- -UUAuGUUUUuaUCGGAGGcuUCUCCG- -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 67365 | 0.72 | 0.994083 |
Target: 5'- uGUugGcccuUGGCCUCCGAcGAGGUu -3' miRNA: 3'- uUAugUuuuuAUCGGAGGCUuCUCCG- -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 74080 | 0.73 | 0.990776 |
Target: 5'- uGAUACAAAAAaauGcCCUCCGuAGAGGg -3' miRNA: 3'- -UUAUGUUUUUau-C-GGAGGCuUCUCCg -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 28817 | 0.73 | 0.989397 |
Target: 5'- uAUACAAAAAUAGgCUCCGuAGAGc- -3' miRNA: 3'- uUAUGUUUUUAUCgGAGGCuUCUCcg -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 4416 | 0.73 | 0.987863 |
Target: 5'- --gACGAAAA-GGCCUCUucGGAGGCc -3' miRNA: 3'- uuaUGUUUUUaUCGGAGGcuUCUCCG- -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 134151 | 0.74 | 0.979957 |
Target: 5'- --aACAAAAAUGcCCUCCGuAGAGGg -3' miRNA: 3'- uuaUGUUUUUAUcGGAGGCuUCUCCg -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 64277 | 0.74 | 0.974799 |
Target: 5'- aAAUGCcugaaguuAAAUAGCCUCUucGGAGGCu -3' miRNA: 3'- -UUAUGuu------UUUAUCGGAGGcuUCUCCG- -5' |
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22834 | 3' | -45.4 | NC_005135.1 | + | 16254 | 0.78 | 0.90375 |
Target: 5'- --aACAAAAAgacCCUCCGAAGAGGg -3' miRNA: 3'- uuaUGUUUUUaucGGAGGCUUCUCCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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