Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22862 | 3' | -54.2 | NC_005137.2 | + | 36529 | 0.66 | 0.922932 |
Target: 5'- cGCACG--UGCAcgACGGCaucGUugACCACCAu -3' miRNA: 3'- -UGUGCuaACGU--UGCCGa--CG--UGGUGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 42246 | 0.66 | 0.933781 |
Target: 5'- aGCACGcGUUGCcucucaacuacAACGaGCUGCGucCCAUCAa -3' miRNA: 3'- -UGUGC-UAACG-----------UUGC-CGACGU--GGUGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 20383 | 0.66 | 0.933781 |
Target: 5'- gGCGCGuuugUGC-ACGGCcGuCACCACgGa -3' miRNA: 3'- -UGUGCua--ACGuUGCCGaC-GUGGUGgU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 78551 | 0.66 | 0.932219 |
Target: 5'- -aACGGUUGCGGCGGagGCGCUugaaacggaagcgcGCCGu -3' miRNA: 3'- ugUGCUAACGUUGCCgaCGUGG--------------UGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 13266 | 0.67 | 0.8695 |
Target: 5'- uAUAgGccgGCGugGGUaGCACCGCCAg -3' miRNA: 3'- -UGUgCuaaCGUugCCGaCGUGGUGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 80238 | 0.67 | 0.883601 |
Target: 5'- uGCGCGGUUGCGuuugaugACGGC-GCgaaCGCCGu -3' miRNA: 3'- -UGUGCUAACGU-------UGCCGaCGug-GUGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 28062 | 0.67 | 0.88432 |
Target: 5'- cGCugGGaUGCGccagACGGCUGUACaCGCa- -3' miRNA: 3'- -UGugCUaACGU----UGCCGACGUG-GUGgu -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 33728 | 0.67 | 0.88432 |
Target: 5'- aACACGuuggcuacUGCGGCGGC-GUugUACCGa -3' miRNA: 3'- -UGUGCua------ACGUUGCCGaCGugGUGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 10862 | 0.67 | 0.891374 |
Target: 5'- cACACGcuGUcGUAACGGCUGCAC-ACg- -3' miRNA: 3'- -UGUGC--UAaCGUUGCCGACGUGgUGgu -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 77376 | 0.67 | 0.898185 |
Target: 5'- aACugGAccgcUUGCAACGagUGCGCCACa- -3' miRNA: 3'- -UGugCU----AACGUUGCcgACGUGGUGgu -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 59884 | 0.67 | 0.88432 |
Target: 5'- aAUACGAggUGCAACaGCuuauagaaUGgGCCGCCAa -3' miRNA: 3'- -UGUGCUa-ACGUUGcCG--------ACgUGGUGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 121838 | 0.67 | 0.8695 |
Target: 5'- aACAgGGUUGCAugGccaacGCUGUugCACUu -3' miRNA: 3'- -UGUgCUAACGUugC-----CGACGugGUGGu -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 31698 | 0.68 | 0.845581 |
Target: 5'- --uUGAUgGCAGCGGCccGC-CCACCAu -3' miRNA: 3'- uguGCUAaCGUUGCCGa-CGuGGUGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 40295 | 0.68 | 0.861746 |
Target: 5'- uCACG-UUGC--UGGCacGCACCACCAg -3' miRNA: 3'- uGUGCuAACGuuGCCGa-CGUGGUGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 75977 | 0.68 | 0.853771 |
Target: 5'- cGCGCGuuggUGC-ACGGCgcacggGCcauuGCCACCAc -3' miRNA: 3'- -UGUGCua--ACGuUGCCGa-----CG----UGGUGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 57411 | 0.68 | 0.853771 |
Target: 5'- -aACGAcuucgUUGCGcCGGCUGCAaaGCCGu -3' miRNA: 3'- ugUGCU-----AACGUuGCCGACGUggUGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 107500 | 0.68 | 0.845581 |
Target: 5'- aACACGuUUGUggUGGCguuugcGCACC-CCAc -3' miRNA: 3'- -UGUGCuAACGuuGCCGa-----CGUGGuGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 97990 | 0.68 | 0.837184 |
Target: 5'- gGCGCGcg-GCGugGG-UGCGCCcGCCAa -3' miRNA: 3'- -UGUGCuaaCGUugCCgACGUGG-UGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 106668 | 0.68 | 0.836333 |
Target: 5'- uACAUGAgcuuUUGCAaccgacaACGGCcgUGCccGCCACCAc -3' miRNA: 3'- -UGUGCU----AACGU-------UGCCG--ACG--UGGUGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 90604 | 0.69 | 0.782924 |
Target: 5'- aACACGAUcucGCAAucggUGGCUGCACaaauccacaGCCAa -3' miRNA: 3'- -UGUGCUAa--CGUU----GCCGACGUGg--------UGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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