Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22862 | 3' | -54.2 | NC_005137.2 | + | 113517 | 1.08 | 0.003637 |
Target: 5'- aACACGAUUGCAACGGCUGCACCACCAc -3' miRNA: 3'- -UGUGCUAACGUUGCCGACGUGGUGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 72336 | 0.76 | 0.418728 |
Target: 5'- aACGCGaAUUGC-GCGaGCUGCGCCGCUc -3' miRNA: 3'- -UGUGC-UAACGuUGC-CGACGUGGUGGu -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 32554 | 0.74 | 0.504792 |
Target: 5'- cAUACGGccgUGCAcGCGGCUGCuugcgcuuuaACCGCCAa -3' miRNA: 3'- -UGUGCUa--ACGU-UGCCGACG----------UGGUGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 85553 | 0.74 | 0.535174 |
Target: 5'- -aACGcgUGC-GCGuGCUGCGCCGCCc -3' miRNA: 3'- ugUGCuaACGuUGC-CGACGUGGUGGu -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 61832 | 0.73 | 0.545446 |
Target: 5'- aGCGCGucuUUGCcgAGCGuGuCUGCGCCACCGu -3' miRNA: 3'- -UGUGCu--AACG--UUGC-C-GACGUGGUGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 84947 | 0.73 | 0.597615 |
Target: 5'- cGCACGcugUGCAGCGGCgGCAUaACCu -3' miRNA: 3'- -UGUGCua-ACGUUGCCGaCGUGgUGGu -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 40825 | 0.72 | 0.618724 |
Target: 5'- aGCGCGuuugugagaAUUGUAACGcGUUGCACgCGCCAa -3' miRNA: 3'- -UGUGC---------UAACGUUGC-CGACGUG-GUGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 112399 | 0.72 | 0.650447 |
Target: 5'- uGCGCGAUcGCGcACGGC-GCGCCAUUg -3' miRNA: 3'- -UGUGCUAaCGU-UGCCGaCGUGGUGGu -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 22443 | 0.7 | 0.723476 |
Target: 5'- -gGCGAUuuggGCAAcgugcgcuccguCGGCUGCACCGCg- -3' miRNA: 3'- ugUGCUAa---CGUU------------GCCGACGUGGUGgu -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 105491 | 0.7 | 0.753715 |
Target: 5'- cGCGCG-UUGCAACGaGCgugacgGCGCCAUg- -3' miRNA: 3'- -UGUGCuAACGUUGC-CGa-----CGUGGUGgu -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 123971 | 0.7 | 0.757675 |
Target: 5'- aAUGCGAUUGCGGCGacgaggccgugacgcGCgUGCGCCACg- -3' miRNA: 3'- -UGUGCUAACGUUGC---------------CG-ACGUGGUGgu -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 105360 | 0.69 | 0.773319 |
Target: 5'- uCGCGAUUGUAGCaGUUGCGCgACa- -3' miRNA: 3'- uGUGCUAACGUUGcCGACGUGgUGgu -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 117248 | 0.69 | 0.773319 |
Target: 5'- cCACGGUUGCcgcugaacauGACGcGCUGCACgGCg- -3' miRNA: 3'- uGUGCUAACG----------UUGC-CGACGUGgUGgu -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 65583 | 0.69 | 0.782924 |
Target: 5'- uACACGg--GCAACGGCauuggGUGCCGCUc -3' miRNA: 3'- -UGUGCuaaCGUUGCCGa----CGUGGUGGu -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 90604 | 0.69 | 0.782924 |
Target: 5'- aACACGAUcucGCAAucggUGGCUGCACaaauccacaGCCAa -3' miRNA: 3'- -UGUGCUAa--CGUU----GCCGACGUGg--------UGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 50625 | 0.69 | 0.792384 |
Target: 5'- uACACGccgGCAcGgGGCUGCAgCACCu -3' miRNA: 3'- -UGUGCuaaCGU-UgCCGACGUgGUGGu -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 59630 | 0.69 | 0.801691 |
Target: 5'- aGCGCGccacuuuUUGCAACGGCa-CGCCGCUg -3' miRNA: 3'- -UGUGCu------AACGUUGCCGacGUGGUGGu -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 39811 | 0.69 | 0.819802 |
Target: 5'- aACACGcgUGCAACcaaacGCcGCGCCACgGa -3' miRNA: 3'- -UGUGCuaACGUUGc----CGaCGUGGUGgU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 88579 | 0.69 | 0.823339 |
Target: 5'- uGCGCGcg-GCGGacacaunacguguccCGGCgggGCACCGCCGa -3' miRNA: 3'- -UGUGCuaaCGUU---------------GCCGa--CGUGGUGGU- -5' |
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22862 | 3' | -54.2 | NC_005137.2 | + | 106668 | 0.68 | 0.836333 |
Target: 5'- uACAUGAgcuuUUGCAaccgacaACGGCcgUGCccGCCACCAc -3' miRNA: 3'- -UGUGCU----AACGU-------UGCCG--ACG--UGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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