Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22873 | 3' | -49.1 | NC_005137.2 | + | 70323 | 0.67 | 0.993646 |
Target: 5'- -aGGUGUUgg-CGGA--GGCGUCGGCg -3' miRNA: 3'- gcUCGCAAauaGUUUuaCCGCGGCCG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 78653 | 0.67 | 0.995323 |
Target: 5'- gCGAGUug-UGUC-AAAUGcGUGUCGGCu -3' miRNA: 3'- -GCUCGcaaAUAGuUUUAC-CGCGGCCG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 63000 | 0.67 | 0.996014 |
Target: 5'- -cGGCGgacc-CAugGUgcGGCGCCGGCg -3' miRNA: 3'- gcUCGCaaauaGUuuUA--CCGCGGCCG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 113667 | 0.66 | 0.997605 |
Target: 5'- -uAGUGUacuauuuaUAUCAGAGUGGUGCgCGuGCg -3' miRNA: 3'- gcUCGCAa-------AUAGUUUUACCGCG-GC-CG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 61373 | 0.74 | 0.827234 |
Target: 5'- uGGGCGgccggCGAguuGAUGGCGuuGGCg -3' miRNA: 3'- gCUCGCaaauaGUU---UUACCGCggCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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