Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22873 | 3' | -49.1 | NC_005137.2 | + | 101868 | 0.71 | 0.949222 |
Target: 5'- aCGcAGCaauuguUUUGUCu---UGGCGCCGGCc -3' miRNA: 3'- -GC-UCGc-----AAAUAGuuuuACCGCGGCCG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 113667 | 0.66 | 0.997605 |
Target: 5'- -uAGUGUacuauuuaUAUCAGAGUGGUGCgCGuGCg -3' miRNA: 3'- gcUCGCAa-------AUAGUUUUACCGCG-GC-CG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 63000 | 0.67 | 0.996014 |
Target: 5'- -cGGCGgacc-CAugGUgcGGCGCCGGCg -3' miRNA: 3'- gcUCGCaaauaGUuuUA--CCGCGGCCG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 78653 | 0.67 | 0.995323 |
Target: 5'- gCGAGUug-UGUC-AAAUGcGUGUCGGCu -3' miRNA: 3'- -GCUCGcaaAUAGuUUUAC-CGCGGCCG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 70323 | 0.67 | 0.993646 |
Target: 5'- -aGGUGUUgg-CGGA--GGCGUCGGCg -3' miRNA: 3'- gcUCGCAAauaGUUUuaCCGCGGCCG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 6997 | 0.67 | 0.992749 |
Target: 5'- aCGAGCGgucgccgggcaCAAcGUGGCGCaCGcGCg -3' miRNA: 3'- -GCUCGCaaaua------GUUuUACCGCG-GC-CG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 35553 | 0.67 | 0.992643 |
Target: 5'- -cGGCGcUUGUCAAGAcacgcacCGCCGGCg -3' miRNA: 3'- gcUCGCaAAUAGUUUUacc----GCGGCCG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 67298 | 0.68 | 0.991519 |
Target: 5'- -aAGCGUUUGUCucuGUuGCGCUGcGCa -3' miRNA: 3'- gcUCGCAAAUAGuuuUAcCGCGGC-CG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 74449 | 0.68 | 0.991519 |
Target: 5'- gGGGCGUUU----GAAUGuGC-CCGGCa -3' miRNA: 3'- gCUCGCAAAuaguUUUAC-CGcGGCCG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 97969 | 0.68 | 0.990262 |
Target: 5'- --cGCGUcaUUGUaggcaAAAcgGGCGCgCGGCg -3' miRNA: 3'- gcuCGCA--AAUAg----UUUuaCCGCG-GCCG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 61373 | 0.74 | 0.827234 |
Target: 5'- uGGGCGgccggCGAguuGAUGGCGuuGGCg -3' miRNA: 3'- gCUCGCaaauaGUU---UUACCGCggCCG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 67725 | 0.65 | 0.998307 |
Target: 5'- gCGAGCGacgg-CGAGAccguguuggaguuUGGCGCCGa- -3' miRNA: 3'- -GCUCGCaaauaGUUUU-------------ACCGCGGCcg -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 82948 | 0.66 | 0.998 |
Target: 5'- -aAGCG----UCGAGAUGGCacGCgCGGCa -3' miRNA: 3'- gcUCGCaaauAGUUUUACCG--CG-GCCG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 1334 | 0.66 | 0.997605 |
Target: 5'- gGAGgaGUUa---AAGGUGGCGgCGGCg -3' miRNA: 3'- gCUCg-CAAauagUUUUACCGCgGCCG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 116340 | 0.67 | 0.996014 |
Target: 5'- aCGGGCGUUUacGUCAAGcuuaaacgauuuGUGcGCGCUGa- -3' miRNA: 3'- -GCUCGCAAA--UAGUUU------------UAC-CGCGGCcg -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 58690 | 0.67 | 0.996014 |
Target: 5'- --cGCGU---UCGGAcaGUGGCGCCGcGUu -3' miRNA: 3'- gcuCGCAaauAGUUU--UACCGCGGC-CG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 104764 | 0.67 | 0.99561 |
Target: 5'- gGGGCG-UUGUUAAcguuccacgagguucGGUGGCccaGUCGGCg -3' miRNA: 3'- gCUCGCaAAUAGUU---------------UUACCG---CGGCCG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 128560 | 0.68 | 0.990262 |
Target: 5'- aGAGUG---GUCGucg-GGCGCgGGCa -3' miRNA: 3'- gCUCGCaaaUAGUuuuaCCGCGgCCG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 87160 | 0.68 | 0.991519 |
Target: 5'- cCGcGGCGUUUcgcacgugcaaAUCGcguucGAcgGGCGCgGGCg -3' miRNA: 3'- -GC-UCGCAAA-----------UAGU-----UUuaCCGCGgCCG- -5' |
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22873 | 3' | -49.1 | NC_005137.2 | + | 93556 | 0.69 | 0.983722 |
Target: 5'- uCGAGCG-----CAAAuacGUGGUGCgCGGCg -3' miRNA: 3'- -GCUCGCaaauaGUUU---UACCGCG-GCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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