Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22897 | 3' | -59.2 | NC_005137.2 | + | 91194 | 0.66 | 0.767641 |
Target: 5'- gGCgCGGCGU-UUGGU---UGCACGCGu -3' miRNA: 3'- gCG-GCCGCAcAGCCGcuuACGUGCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 34601 | 0.66 | 0.777734 |
Target: 5'- uCGCCGcGCGgguagcgGUCGGCGcggcuccauauuuuuAUGUugGCc -3' miRNA: 3'- -GCGGC-CGCa------CAGCCGCu--------------UACGugCGc -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 126678 | 0.66 | 0.782273 |
Target: 5'- gCGCuCGGUGUccgggcauguuaaUCGGCGugcauUGCACGCa -3' miRNA: 3'- -GCG-GCCGCAc------------AGCCGCuu---ACGUGCGc -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 26564 | 0.66 | 0.785881 |
Target: 5'- gCGCugCGGCGUGUCGacuGCcaaGAUGUacACGCGg -3' miRNA: 3'- -GCG--GCCGCACAGC---CGc--UUACG--UGCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 124967 | 0.66 | 0.785881 |
Target: 5'- gCGCCGGUGaUGg-GGCGc-UGCGCuGCGu -3' miRNA: 3'- -GCGGCCGC-ACagCCGCuuACGUG-CGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 114255 | 0.66 | 0.776823 |
Target: 5'- aGCCGGac--UUGGCGggUcGCACGgGg -3' miRNA: 3'- gCGGCCgcacAGCCGCuuA-CGUGCgC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 46439 | 0.66 | 0.77591 |
Target: 5'- cCGCUGGCGcacaugcUGU-GGCacAUGCACGCc -3' miRNA: 3'- -GCGGCCGC-------ACAgCCGcuUACGUGCGc -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 32901 | 0.66 | 0.747992 |
Target: 5'- aGCCGGCGg--CGGUGAcaaaacucgcaacAUGCagACGUGc -3' miRNA: 3'- gCGGCCGCacaGCCGCU-------------UACG--UGCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 125757 | 0.66 | 0.767641 |
Target: 5'- gCGCCGcGCGUuUCGaGCG---GCGCGUGg -3' miRNA: 3'- -GCGGC-CGCAcAGC-CGCuuaCGUGCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 53100 | 0.66 | 0.767641 |
Target: 5'- gGUgGGUGUGUUuGCGGuUGCACGaCGc -3' miRNA: 3'- gCGgCCGCACAGcCGCUuACGUGC-GC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 129507 | 0.66 | 0.758343 |
Target: 5'- gCGUCGGCGccgcgCGGCGgAAUGUAC-CGg -3' miRNA: 3'- -GCGGCCGCaca--GCCGC-UUACGUGcGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 80395 | 0.66 | 0.758343 |
Target: 5'- uGCCGGCGUGUauGa----GUACGCGu -3' miRNA: 3'- gCGGCCGCACAgcCgcuuaCGUGCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 70194 | 0.67 | 0.700611 |
Target: 5'- uGuuGGUGgaggcGUCGGUGAagGUGUugGUGa -3' miRNA: 3'- gCggCCGCa----CAGCCGCU--UACGugCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 10935 | 0.67 | 0.694698 |
Target: 5'- cCGCCGGCGaguucaccaucacgUcaauaggcacuucgGUCGcGUGAAUaGCGCGCGa -3' miRNA: 3'- -GCGGCCGC--------------A--------------CAGC-CGCUUA-CGUGCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 117580 | 0.68 | 0.640881 |
Target: 5'- cCGCCGGUGcGgcugacgGGCGAcGUGUACGCu -3' miRNA: 3'- -GCGGCCGCaCag-----CCGCU-UACGUGCGc -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 2692 | 0.68 | 0.670882 |
Target: 5'- uGCCGGUGg--CGGCGAAUuaaGCACa-- -3' miRNA: 3'- gCGGCCGCacaGCCGCUUA---CGUGcgc -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 20195 | 0.68 | 0.650898 |
Target: 5'- gCGUCGGCGgg-CGGCGucgauUGCgaaaGCGCGu -3' miRNA: 3'- -GCGGCCGCacaGCCGCuu---ACG----UGCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 129003 | 0.68 | 0.630856 |
Target: 5'- gCGCCGcaGCGUGcaCGaGCu-GUGCACGCGg -3' miRNA: 3'- -GCGGC--CGCACa-GC-CGcuUACGUGCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 95575 | 0.68 | 0.630856 |
Target: 5'- aCGgCaGCGUGUUGGCGuuguaguuaauGAUGgGCGCGc -3' miRNA: 3'- -GCgGcCGCACAGCCGC-----------UUACgUGCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 20564 | 0.68 | 0.620833 |
Target: 5'- gCGCCGGCcg--CGGUG--UGCACGCu -3' miRNA: 3'- -GCGGCCGcacaGCCGCuuACGUGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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