Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22897 | 3' | -59.2 | NC_005137.2 | + | 385 | 0.69 | 0.600818 |
Target: 5'- uGCUGGCuuUGUCGGCGcgcGUGUcCGCGc -3' miRNA: 3'- gCGGCCGc-ACAGCCGCu--UACGuGCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 2692 | 0.68 | 0.670882 |
Target: 5'- uGCCGGUGg--CGGCGAAUuaaGCACa-- -3' miRNA: 3'- gCGGCCGCacaGCCGCUUA---CGUGcgc -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 10935 | 0.67 | 0.694698 |
Target: 5'- cCGCCGGCGaguucaccaucacgUcaauaggcacuucgGUCGcGUGAAUaGCGCGCGa -3' miRNA: 3'- -GCGGCCGC--------------A--------------CAGC-CGCUUA-CGUGCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 20195 | 0.68 | 0.650898 |
Target: 5'- gCGUCGGCGgg-CGGCGucgauUGCgaaaGCGCGu -3' miRNA: 3'- -GCGGCCGCacaGCCGCuu---ACG----UGCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 20564 | 0.68 | 0.620833 |
Target: 5'- gCGCCGGCcg--CGGUG--UGCACGCu -3' miRNA: 3'- -GCGGCCGcacaGCCGCuuACGUGCGc -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 26564 | 0.66 | 0.785881 |
Target: 5'- gCGCugCGGCGUGUCGacuGCcaaGAUGUacACGCGg -3' miRNA: 3'- -GCG--GCCGCACAGC---CGc--UUACG--UGCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 32901 | 0.66 | 0.747992 |
Target: 5'- aGCCGGCGg--CGGUGAcaaaacucgcaacAUGCagACGUGc -3' miRNA: 3'- gCGGCCGCacaGCCGCU-------------UACG--UGCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 34601 | 0.66 | 0.777734 |
Target: 5'- uCGCCGcGCGgguagcgGUCGGCGcggcuccauauuuuuAUGUugGCc -3' miRNA: 3'- -GCGGC-CGCa------CAGCCGCu--------------UACGugCGc -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 34709 | 1.08 | 0.001648 |
Target: 5'- gCGCCGGCGUGUCGGCGAAUGCACGCGu -3' miRNA: 3'- -GCGGCCGCACAGCCGCUUACGUGCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 46439 | 0.66 | 0.77591 |
Target: 5'- cCGCUGGCGcacaugcUGU-GGCacAUGCACGCc -3' miRNA: 3'- -GCGGCCGC-------ACAgCCGcuUACGUGCGc -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 53100 | 0.66 | 0.767641 |
Target: 5'- gGUgGGUGUGUUuGCGGuUGCACGaCGc -3' miRNA: 3'- gCGgCCGCACAGcCGCUuACGUGC-GC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 63018 | 0.69 | 0.610818 |
Target: 5'- gCGCCGGCGUGUCGGC-AA-GCu---- -3' miRNA: 3'- -GCGGCCGCACAGCCGcUUaCGugcgc -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 70155 | 0.74 | 0.333335 |
Target: 5'- aGgUGGCgGUGUCGGUGAagGUGUugGCGu -3' miRNA: 3'- gCgGCCG-CACAGCCGCU--UACGugCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 70194 | 0.67 | 0.700611 |
Target: 5'- uGuuGGUGgaggcGUCGGUGAagGUGUugGUGa -3' miRNA: 3'- gCggCCGCa----CAGCCGCU--UACGugCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 70241 | 0.69 | 0.568013 |
Target: 5'- gCGUCGGUGaaggcGUCGGUGAaggcgucgguaaagGUGUugGCGg -3' miRNA: 3'- -GCGGCCGCa----CAGCCGCU--------------UACGugCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 70289 | 0.69 | 0.568013 |
Target: 5'- gCGUCGGUGaaggcGUCGGUGAaggcgucgguaaagGUGUugGCGg -3' miRNA: 3'- -GCGGCCGCa----CAGCCGCU--------------UACGugCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 70337 | 0.76 | 0.23618 |
Target: 5'- gCGUCGGCGaaggcGUCGGUGAagGUGUugGCGa -3' miRNA: 3'- -GCGGCCGCa----CAGCCGCU--UACGugCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 70413 | 0.72 | 0.421428 |
Target: 5'- uGuuGGCGaaggcGUCGGUGAagGUGUugGCGg -3' miRNA: 3'- gCggCCGCa----CAGCCGCU--UACGugCGC- -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 75125 | 0.71 | 0.475018 |
Target: 5'- aGUCGGCGUcGUCGGCGcgauuguUGgACGCc -3' miRNA: 3'- gCGGCCGCA-CAGCCGCuu-----ACgUGCGc -5' |
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22897 | 3' | -59.2 | NC_005137.2 | + | 80395 | 0.66 | 0.758343 |
Target: 5'- uGCCGGCGUGUauGa----GUACGCGu -3' miRNA: 3'- gCGGCCGCACAgcCgcuuaCGUGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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