Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22916 | 5' | -53.6 | NC_005137.2 | + | 100189 | 0.66 | 0.949534 |
Target: 5'- cGCGCGCCGcgUcggaccGCGUGCuuGcGCAc -3' miRNA: 3'- aCGUGCGGCuaAu-----UGUACGggCcUGU- -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 64157 | 0.66 | 0.945083 |
Target: 5'- cGCAC-CCGugcgcguUUAACG-GCgCCGGACAc -3' miRNA: 3'- aCGUGcGGCu------AAUUGUaCG-GGCCUGU- -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 61415 | 0.66 | 0.945083 |
Target: 5'- --aACGCCGcgUucucaAACAUGUCgGGACGu -3' miRNA: 3'- acgUGCGGCuaA-----UUGUACGGgCCUGU- -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 114239 | 0.66 | 0.940382 |
Target: 5'- uUGCACGCCGu---GCAauagCCGGACu -3' miRNA: 3'- -ACGUGCGGCuaauUGUacg-GGCCUGu -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 74870 | 0.66 | 0.930222 |
Target: 5'- cUGCGaagUGCCGcugGACGUGUUgGGACAa -3' miRNA: 3'- -ACGU---GCGGCuaaUUGUACGGgCCUGU- -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 7623 | 0.67 | 0.92476 |
Target: 5'- aUGCAuuucagaguuuCGCCGGccAGCGUGCCCaGAUu -3' miRNA: 3'- -ACGU-----------GCGGCUaaUUGUACGGGcCUGu -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 75232 | 0.67 | 0.92476 |
Target: 5'- cUGCugGCCGAaaugaUGGcCGUGaCCgCGGGCGc -3' miRNA: 3'- -ACGugCGGCUa----AUU-GUAC-GG-GCCUGU- -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 7476 | 0.67 | 0.919044 |
Target: 5'- gGgGCGCCagcacuUUGACcgcauccUGCCCGGGCAa -3' miRNA: 3'- aCgUGCGGcu----AAUUGu------ACGGGCCUGU- -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 29515 | 0.67 | 0.913074 |
Target: 5'- aGCACGCCaGUUuuuguGgGUGCgCCGcGACAa -3' miRNA: 3'- aCGUGCGGcUAAu----UgUACG-GGC-CUGU- -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 95754 | 0.67 | 0.906851 |
Target: 5'- aGCACGUCGcgcacaGUGCgCGGGCGg -3' miRNA: 3'- aCGUGCGGCuaauugUACGgGCCUGU- -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 110727 | 0.67 | 0.900379 |
Target: 5'- gUGCGCGCaGAgcgcuUGUGCgCCGGGCGa -3' miRNA: 3'- -ACGUGCGgCUaauu-GUACG-GGCCUGU- -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 31158 | 0.68 | 0.893658 |
Target: 5'- aGCgGCGCCGAccAGCGUGCgCGGuuGg -3' miRNA: 3'- aCG-UGCGGCUaaUUGUACGgGCCugU- -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 99596 | 0.68 | 0.879491 |
Target: 5'- aGCcCGCCGGU----GUGCCUGGugGc -3' miRNA: 3'- aCGuGCGGCUAauugUACGGGCCugU- -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 56669 | 0.68 | 0.872053 |
Target: 5'- gUGCuuGCCGAcggcgcugugGACGUGaCCGGACGc -3' miRNA: 3'- -ACGugCGGCUaa--------UUGUACgGGCCUGU- -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 115289 | 0.68 | 0.872053 |
Target: 5'- gUGC-CGCCGGUc-ACGUGCCgcccgaauuUGGACAc -3' miRNA: 3'- -ACGuGCGGCUAauUGUACGG---------GCCUGU- -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 76658 | 0.68 | 0.864386 |
Target: 5'- gUGCACGCCGuugAAUAgacGCCCGuGuCAu -3' miRNA: 3'- -ACGUGCGGCuaaUUGUa--CGGGC-CuGU- -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 95776 | 0.68 | 0.862042 |
Target: 5'- cGCACGUCGG--GACGUuugauuagccacacGaCCCGGACGg -3' miRNA: 3'- aCGUGCGGCUaaUUGUA--------------C-GGGCCUGU- -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 96663 | 0.69 | 0.856496 |
Target: 5'- uUGCGCGCCGcuaguCAUagcGCCCguGGACAc -3' miRNA: 3'- -ACGUGCGGCuaauuGUA---CGGG--CCUGU- -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 97373 | 0.69 | 0.843426 |
Target: 5'- aGCAUGCUGcgcGACAUGCuguacgacgacagcaCCGGGCAg -3' miRNA: 3'- aCGUGCGGCuaaUUGUACG---------------GGCCUGU- -5' |
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22916 | 5' | -53.6 | NC_005137.2 | + | 52020 | 0.69 | 0.840075 |
Target: 5'- uUGuCGCGCCGGgcgUAaaagGCGUGCacaUGGACAa -3' miRNA: 3'- -AC-GUGCGGCUa--AU----UGUACGg--GCCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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