Results 1 - 20 of 70 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22917 | 3' | -46.3 | NC_005137.2 | + | 35559 | 0.67 | 0.999357 |
Target: 5'- uUGUCAAGacacGCACCGccGGCGccauuuugauaaACGCu -3' miRNA: 3'- cAUAGUUUaa--CGUGGCuuCCGU------------UGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 130304 | 0.69 | 0.997384 |
Target: 5'- aUGUCAGAgccGCgcaaaggcgcuACCGAucGCAGCGCg -3' miRNA: 3'- cAUAGUUUaa-CG-----------UGGCUucCGUUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 96396 | 0.68 | 0.998392 |
Target: 5'- aUAUgGAGccGCGCCGAccgcuacccgcgcGGCGACGCg -3' miRNA: 3'- cAUAgUUUaaCGUGGCUu------------CCGUUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 34600 | 0.68 | 0.998513 |
Target: 5'- uGUAUCAAA--GCACCGAAuuGGCgGACa- -3' miRNA: 3'- -CAUAGUUUaaCGUGGCUU--CCG-UUGcg -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 68471 | 0.68 | 0.998513 |
Target: 5'- -cGUCGAccGUUGCgacaacacGCCGggGGauacaAGCGCc -3' miRNA: 3'- caUAGUU--UAACG--------UGGCuuCCg----UUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 32512 | 0.68 | 0.998783 |
Target: 5'- -cAUCGGGUUGUGCUGGaugAGGCucACGUc -3' miRNA: 3'- caUAGUUUAACGUGGCU---UCCGu-UGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 95484 | 0.68 | 0.998783 |
Target: 5'- -gGUCGGAUgcGCGCCGcacGGGCGcauCGCu -3' miRNA: 3'- caUAGUUUAa-CGUGGCu--UCCGUu--GCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 71363 | 0.68 | 0.99901 |
Target: 5'- -cGUCGGccgcGUUG-ACUGucGGCGACGCg -3' miRNA: 3'- caUAGUU----UAACgUGGCuuCCGUUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 46647 | 0.68 | 0.9992 |
Target: 5'- ---aCAGcg-GCGCUaAGGGCAGCGCc -3' miRNA: 3'- cauaGUUuaaCGUGGcUUCCGUUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 95647 | 0.69 | 0.995616 |
Target: 5'- cGUG-CGccgUGCGCCGAcGGGCAaguGCGCc -3' miRNA: 3'- -CAUaGUuuaACGUGGCU-UCCGU---UGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 102249 | 0.7 | 0.991858 |
Target: 5'- -aGUCAAugaccggUGUGCC--AGGCGACGCg -3' miRNA: 3'- caUAGUUua-----ACGUGGcuUCCGUUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 51235 | 0.7 | 0.991858 |
Target: 5'- gGUGUUGGAcUGCAaaGggGGCAaaucguagcgGCGCa -3' miRNA: 3'- -CAUAGUUUaACGUggCuuCCGU----------UGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 95137 | 0.82 | 0.586524 |
Target: 5'- -gGUgAGAUUGuCAUUGAGGGCAACGCg -3' miRNA: 3'- caUAgUUUAAC-GUGGCUUCCGUUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 11627 | 0.74 | 0.943617 |
Target: 5'- ----uGGAUaGCACCGAGcucGGCGGCGCg -3' miRNA: 3'- cauagUUUAaCGUGGCUU---CCGUUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 64808 | 0.74 | 0.95288 |
Target: 5'- ---aCAug-UGCACCGAaucGGGCGACGg -3' miRNA: 3'- cauaGUuuaACGUGGCU---UCCGUUGCg -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 96971 | 0.72 | 0.977088 |
Target: 5'- --cUCAAAUUGCGCCaacaaguAGGCucgauGACGCa -3' miRNA: 3'- cauAGUUUAACGUGGcu-----UCCG-----UUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 99507 | 0.71 | 0.983998 |
Target: 5'- uUGUCAAAUUGCGgCu--GGCcGCGCa -3' miRNA: 3'- cAUAGUUUAACGUgGcuuCCGuUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 34850 | 0.71 | 0.990602 |
Target: 5'- -gAUgGGAUgGUACCGcacggcGGGCAACGCg -3' miRNA: 3'- caUAgUUUAaCGUGGCu-----UCCGUUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 87953 | 0.71 | 0.990602 |
Target: 5'- ---aCAAAUUGacuuaacugguCGCCGuccguGGGCGACGCg -3' miRNA: 3'- cauaGUUUAAC-----------GUGGCu----UCCGUUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 48749 | 0.7 | 0.991858 |
Target: 5'- -------cUUGuCGCCGAacaAGGCGGCGCg -3' miRNA: 3'- cauaguuuAAC-GUGGCU---UCCGUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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