Results 1 - 20 of 70 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22917 | 3' | -46.3 | NC_005137.2 | + | 1101 | 0.69 | 0.99778 |
Target: 5'- ---cCuuAUUGCGCCGAugacGGGCGcuggucuACGCu -3' miRNA: 3'- cauaGuuUAACGUGGCU----UCCGU-------UGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 2520 | 0.71 | 0.985907 |
Target: 5'- ---aCGGAUUGUGCCGucGGguACGUa -3' miRNA: 3'- cauaGUUUAACGUGGCuuCCguUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 4387 | 0.68 | 0.998195 |
Target: 5'- cGUGUCGccgaccgacgcAAUUGCACguuuUGAA-GCAGCGCg -3' miRNA: 3'- -CAUAGU-----------UUAACGUG----GCUUcCGUUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 7661 | 1.14 | 0.010902 |
Target: 5'- aGUAUCAAAUUGCACCGAAGGCAACGCu -3' miRNA: 3'- -CAUAGUUUAACGUGGCUUCCGUUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 7855 | 0.74 | 0.95288 |
Target: 5'- -aAUCAGucaUGCGCCGcAGGCAuuACGUg -3' miRNA: 3'- caUAGUUua-ACGUGGCuUCCGU--UGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 11627 | 0.74 | 0.943617 |
Target: 5'- ----uGGAUaGCACCGAGcucGGCGGCGCg -3' miRNA: 3'- cauagUUUAaCGUGGCUU---CCGUUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 13005 | 0.69 | 0.995897 |
Target: 5'- ---cCAGAUUGCGCCGGGcuacGGCcuggccgaaaacuuuGACGCc -3' miRNA: 3'- cauaGUUUAACGUGGCUU----CCG---------------UUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 27472 | 0.66 | 0.999887 |
Target: 5'- cGUcgCGAcgUGCGCCGccAGuCAAUGCu -3' miRNA: 3'- -CAuaGUUuaACGUGGCu-UCcGUUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 27673 | 0.7 | 0.991858 |
Target: 5'- -gGUCAug-UGCGCgGAAGcuaacccacccuGCAACGCa -3' miRNA: 3'- caUAGUuuaACGUGgCUUC------------CGUUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 28633 | 0.74 | 0.943617 |
Target: 5'- cGUGUCA--UUGCACCaaaacGAccGCAACGCg -3' miRNA: 3'- -CAUAGUuuAACGUGG-----CUucCGUUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 28951 | 0.73 | 0.964756 |
Target: 5'- --uUCGAuagacgugUGCGCCGAGGGC-GCGUu -3' miRNA: 3'- cauAGUUua------ACGUGGCUUCCGuUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 31375 | 0.66 | 0.99975 |
Target: 5'- --uUCAAGcuggUGUGCgCGAccgcugguacaaGGGCGACGCg -3' miRNA: 3'- cauAGUUUa---ACGUG-GCU------------UCCGUUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 32512 | 0.68 | 0.998783 |
Target: 5'- -cAUCGGGUUGUGCUGGaugAGGCucACGUc -3' miRNA: 3'- caUAGUUUAACGUGGCU---UCCGu-UGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 33121 | 0.66 | 0.999887 |
Target: 5'- -gGUCA----GCGCCGGGuGGCAgcacauuugGCGCa -3' miRNA: 3'- caUAGUuuaaCGUGGCUU-CCGU---------UGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 34600 | 0.68 | 0.998513 |
Target: 5'- uGUAUCAAA--GCACCGAAuuGGCgGACa- -3' miRNA: 3'- -CAUAGUUUaaCGUGGCUU--CCG-UUGcg -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 34850 | 0.71 | 0.990602 |
Target: 5'- -gAUgGGAUgGUACCGcacggcGGGCAACGCg -3' miRNA: 3'- caUAgUUUAaCGUGGCu-----UCCGUUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 35122 | 0.67 | 0.999487 |
Target: 5'- -gGUCAA----CACCGAAGGUuugacACGCg -3' miRNA: 3'- caUAGUUuaacGUGGCUUCCGu----UGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 35559 | 0.67 | 0.999357 |
Target: 5'- uUGUCAAGacacGCACCGccGGCGccauuuugauaaACGCu -3' miRNA: 3'- cAUAGUUUaa--CGUGGCuuCCGU------------UGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 35724 | 0.7 | 0.994763 |
Target: 5'- uUAUCAAAaugGCGCCGgcGGUgcgugucuugacaAGCGCc -3' miRNA: 3'- cAUAGUUUaa-CGUGGCuuCCG-------------UUGCG- -5' |
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22917 | 3' | -46.3 | NC_005137.2 | + | 38079 | 0.66 | 0.999807 |
Target: 5'- aGUGUCAcuGAccGcCACgGAcaagcccacGGGCAACGCg -3' miRNA: 3'- -CAUAGU--UUaaC-GUGgCU---------UCCGUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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