Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22919 | 3' | -52.1 | NC_005137.2 | + | 110729 | 0.85 | 0.187735 |
Target: 5'- aGGAugGGCGCGCCAagacGCAGgugCGGGCg -3' miRNA: 3'- gCCUugCCGCGCGGU----UGUCaa-GUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 21120 | 0.66 | 0.978573 |
Target: 5'- gCGGcaaAAC-GCGCGCCG-CAGgcagCAGACg -3' miRNA: 3'- -GCC---UUGcCGCGCGGUuGUCaa--GUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 18405 | 0.66 | 0.978573 |
Target: 5'- aCGGAcGCGGCGC-C--GCAG-UCGAACg -3' miRNA: 3'- -GCCU-UGCCGCGcGguUGUCaAGUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 22618 | 0.66 | 0.978573 |
Target: 5'- gCGuGAgcGCGGUGCaGCCGACGGagCGcACg -3' miRNA: 3'- -GC-CU--UGCCGCG-CGGUUGUCaaGUuUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 7352 | 0.66 | 0.976095 |
Target: 5'- uGGAagACGGCacGCGCC---GGUUCGAAa -3' miRNA: 3'- gCCU--UGCCG--CGCGGuugUCAAGUUUg -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 54891 | 0.66 | 0.967412 |
Target: 5'- gCGGu-UGuGCGCGCCAACGaUUCGuACa -3' miRNA: 3'- -GCCuuGC-CGCGCGGUUGUcAAGUuUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 41965 | 0.67 | 0.964076 |
Target: 5'- -cGcgUGGCGCGCCGugACAGcgUCAGAa -3' miRNA: 3'- gcCuuGCCGCGCGGU--UGUCa-AGUUUg -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 41612 | 0.67 | 0.964076 |
Target: 5'- gCGGAcaGCGcGUGCGCCAu--GUUUAAAa -3' miRNA: 3'- -GCCU--UGC-CGCGCGGUuguCAAGUUUg -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 131035 | 0.67 | 0.960139 |
Target: 5'- ---uGCGGCGCGUCcGCGGcguacaaUUCAGGCg -3' miRNA: 3'- gccuUGCCGCGCGGuUGUC-------AAGUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 15412 | 0.68 | 0.923082 |
Target: 5'- aCGG-GCGGUGCGUUuu--GUUCGAACg -3' miRNA: 3'- -GCCuUGCCGCGCGGuuguCAAGUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 99704 | 0.69 | 0.898244 |
Target: 5'- uCGGAacaaGCGaGCGCGCUGACGcagUUAAACg -3' miRNA: 3'- -GCCU----UGC-CGCGCGGUUGUca-AGUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 74429 | 0.72 | 0.782424 |
Target: 5'- aGGcAACuGCGCGCCuuguCAGUUgCAAACa -3' miRNA: 3'- gCC-UUGcCGCGCGGuu--GUCAA-GUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 72496 | 0.77 | 0.493095 |
Target: 5'- gCGGAGCGGCGCaGCUcGCGcaauucgcGUUCGGACa -3' miRNA: 3'- -GCCUUGCCGCG-CGGuUGU--------CAAGUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 18484 | 0.66 | 0.970523 |
Target: 5'- ---cGCGGCuGUGCCGACgucaAGUUUAAGCc -3' miRNA: 3'- gccuUGCCG-CGCGGUUG----UCAAGUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 84055 | 0.66 | 0.980855 |
Target: 5'- aCGGAAUaGUGUcaaaGCCAGCGGUUUGuAACa -3' miRNA: 3'- -GCCUUGcCGCG----CGGUUGUCAAGU-UUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 70118 | 0.66 | 0.980855 |
Target: 5'- cCGGAAaguacaugGGCuCGcCCAACGGUUCGGGa -3' miRNA: 3'- -GCCUUg-------CCGcGC-GGUUGUCAAGUUUg -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 100267 | 0.66 | 0.97711 |
Target: 5'- aCGGGcgcgcgucGCGGCauucaCGCCcacaaucuccacauGCAGUUCAAACg -3' miRNA: 3'- -GCCU--------UGCCGc----GCGGu-------------UGUCAAGUUUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 100039 | 0.66 | 0.976095 |
Target: 5'- gCGGuccgacGCGGCGCGC-GGCGcGUUCAGc- -3' miRNA: 3'- -GCCu-----UGCCGCGCGgUUGU-CAAGUUug -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 110700 | 0.66 | 0.973415 |
Target: 5'- uCGGGucauGCGcGCGUGCgCAACGGUgugCGcGCa -3' miRNA: 3'- -GCCU----UGC-CGCGCG-GUUGUCAa--GUuUG- -5' |
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22919 | 3' | -52.1 | NC_005137.2 | + | 63002 | 0.66 | 0.973415 |
Target: 5'- gCGGAcccAUGGUgcgGCGCCGGCGuGUcggCAAGCu -3' miRNA: 3'- -GCCU---UGCCG---CGCGGUUGU-CAa--GUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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