Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22920 | 3' | -54.3 | NC_005137.2 | + | 95655 | 0.66 | 0.929023 |
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Target: 5'- -gUGCGCCGACGgGCAaGugC-GCCUg -3' miRNA: 3'- ugAUGCGGCUGUgCGUaUugGgUGGA- -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 59946 | 0.66 | 0.929023 |
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Target: 5'- aGCUggcGCGCCucguuGAUGCGCAU-GCCCguACCUa -3' miRNA: 3'- -UGA---UGCGG-----CUGUGCGUAuUGGG--UGGA- -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 33351 | 0.66 | 0.934247 |
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Target: 5'- -gUGCGCucuuCGACGCGCAauUGACCCGu-- -3' miRNA: 3'- ugAUGCG----GCUGUGCGU--AUUGGGUgga -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 48888 | 0.66 | 0.934247 |
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Target: 5'- uACUGCGCCG-CGCuGCGgc-UUCGCCUg -3' miRNA: 3'- -UGAUGCGGCuGUG-CGUauuGGGUGGA- -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 70592 | 0.66 | 0.93922 |
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Target: 5'- uGCUGCGCgGGCggcuuuuuguuGCGCugAAUCCACUUg -3' miRNA: 3'- -UGAUGCGgCUG-----------UGCGuaUUGGGUGGA- -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 7847 | 0.66 | 0.93922 |
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Target: 5'- -aUGCGCCG-CAgGCAUuacguGCCCAUg- -3' miRNA: 3'- ugAUGCGGCuGUgCGUAu----UGGGUGga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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