Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22920 | 3' | -54.3 | NC_005137.2 | + | 111538 | 0.69 | 0.785561 |
|
Target: 5'- aGCU--GCUGGCaaGCGCuUGACCCACCa -3' miRNA: 3'- -UGAugCGGCUG--UGCGuAUUGGGUGGa -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 115881 | 0.67 | 0.878316 |
|
Target: 5'- uGCUugGCaaCGACGCGUGUug-CCACCa -3' miRNA: 3'- -UGAugCG--GCUGUGCGUAuugGGUGGa -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 122498 | 0.67 | 0.903715 |
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Target: 5'- aACUGCGUCGcCGCGCGUcuuuuucacgugcaAGCCUGCa- -3' miRNA: 3'- -UGAUGCGGCuGUGCGUA--------------UUGGGUGga -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 122875 | 0.69 | 0.813084 |
|
Target: 5'- uGCUaagcACGCCcaGCACGCGUAACCUuggcaacgGCCg -3' miRNA: 3'- -UGA----UGCGGc-UGUGCGUAUUGGG--------UGGa -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 123118 | 0.67 | 0.905629 |
|
Target: 5'- aGCUGCGCCGguguGCACGUuuu-CCUACg- -3' miRNA: 3'- -UGAUGCGGC----UGUGCGuauuGGGUGga -5' |
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| 22920 | 3' | -54.3 | NC_005137.2 | + | 130772 | 0.68 | 0.855403 |
|
Target: 5'- gGCUuuGUCGGCGCGCGUGuCCgCGCUUu -3' miRNA: 3'- -UGAugCGGCUGUGCGUAUuGG-GUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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